Accedi

Elizabethtown College

2 ARTICLES PUBLISHED IN JoVE

Behavior

Examination of Rapid Dopamine Dynamics with Fast Scan Cyclic Voltammetry During Intra-oral Tastant Administration in Awake Rats

Robert J. Wickham¹, Jinwoo Park², Eric J. Nunes³, Nii A. Addy^1,3,4

¹Interdepartmental Neuroscience Program, Yale University, ²Department of Biotechnical and Clinical Laboratory Sciences, School of Medicine and Biomedical Sciences, University at Buffalo, ³Department of Psychiatry, Yale School of Medicine, ⁴Department of Cellular and Molecular Physiology, Yale School of Medicine

Rapid fluctuations in extracellular dopamine (DA) mediate both reward processing and motivated behavior in mammals. This manuscript describes the combined use of fast scan cyclic voltammetry (FSCV) and intra-oral tastant administration to determine how tastants alter rapid dopamine release in awake, freely moving rats.

Neuroscience

Combined Infusion and Stimulation with Fast-Scan Cyclic Voltammetry (CIS-FSCV) to Assess Ventral Tegmental Area Receptor Regulation of Phasic Dopamine

Robert J. Wickham¹, Makenzie Lehr^*1, Lauryn Mitchell^*1, Nii A. Addy^2,3

¹Department of Psychology, Elizabethtown College, ²Department of Psychiatry, Yale University, ³Department of Cellular and Molecular Physiology, Yale University

The goal of this protocol is to directly manipulate ventral tegmental area receptors to study their contribution to subsecond dopamine release.