Fairfield University

4 ARTICLES PUBLISHED IN JoVE

Behavior

Automated, Quantitative Cognitive/Behavioral Screening of Mice: For Genetics, Pharmacology, Animal Cognition and Undergraduate Instruction

C. R. Gallistel¹, Fuat Balci^1,2, David Freestone^1,3, Aaron Kheifets¹, Adam King⁴

¹Department of Psychology, Rutgers University, ²Department of Psychology, Koç University, ³Center for Neural Science, New York University, ⁴Department of Mathematics & Computer Science, Fairfield University

Fully automated system for measuring physiologically meaningful properties of the mechanisms mediating spatial localization, temporal localization, duration, rate and probability estimation, risk assessment, impulsivity, and the accuracy and precision of memory, in order to assess the effects of genetic and pharmacological manipulations on foundational mechanisms of cognition in mice.

Bioengineering

Encapsulating Cytochrome c in Silica Aerogel Nanoarchitectures without Metal Nanoparticles while Retaining Gas-phase Bioactivity

Amanda S. Harper-Leatherman¹, Elizabeth R. Pacer¹, Nina D. Kosciuszek¹

¹Department of Chemistry & Biochemistry, Fairfield University

This procedure describes how to encapsulate cytochrome c (cyt. c) in silica (SiO₂) sol-gels, process these gels to form bioaerogels, and use these bioaerogels to rapidly recognize nitric oxide (NO) through a gas-phase reaction. This type of protocol may aid in the future development of biosensors or other bioanalytical devices.

Chemistry

Synthesis and Characterization of 1,2-Dithiolane Modified Self-Assembling Peptides

Ruben Neves¹, Kailyn Stephens¹, Jillian E. Smith-Carpenter¹

¹Department of Chemistry and Biochemistry, Fairfield University

A protocol for the synthesis of a 1,2-dithiolane modified peptide and the characterization of the supramolecular structures resulting from the peptide self-assembly.

Education

Preparation of SNS Cobalt(II) Pincer Model Complexes of Liver Alcohol Dehydrogenase

John R. Miecznikowski¹, Jerry P. Jasinski², Manpreet Kaur², Sheila C. Bonitatibus¹, Emilse M. Almanza¹, Rami M. Kharbouch¹, Samantha E. Zygmont¹, Kendra R. Landy¹

¹Department of Chemistry and Biochemistry, Fairfield University, ²Department of Chemistry, Keene State College

The preparation of SNS pincer cobalt(II) model complexes of liver alcohol dehydrogenase is presented here. The complexes can be prepared by reacting the ligand precursor with CoCl₂·6H₂O and can then be recrystallized by allowing diethyl ether to slowly diffuse into an acetonitrile solution that contains the cobalt complex.