April 12th, 2010
•The accuracy and sensitivity of protein determination by the rapid and convenient Bradford assay is compromised by intrinsic nonlinearity. We show a simple linearization procedure that greatly increases the accuracy, improves the sensitivity of the assay about 10-fold, and significantly reduces interference by detergents.
Related Videos
Actin Co-Sedimentation Assay; for the Analysis of Protein Binding to F-Actin
Detection of Protein Ubiquitination
Staining of Proteins in Gels with Coomassie G-250 without Organic Solvent and Acetic Acid
Using the GELFREE 8100 Fractionation System for Molecular Weight-Based Fractionation with Liquid Phase Recovery
Bimolecular Fluorescence Complementation (BiFC) Assay for Protein-Protein Interaction in Onion Cells Using the Helios Gene Gun
A Liquid Phase Affinity Capture Assay Using Magnetic Beads to Study Protein-Protein Interaction: The Poliovirus-Nanobody Example
Linking Predation Risk, Herbivore Physiological Stress and Microbial Decomposition of Plant Litter
Reporter-based Growth Assay for Systematic Analysis of Protein Degradation
Quantitative Immunofluorescence Assay to Measure the Variation in Protein Levels at Centrosomes
Identifying Protein-protein Interaction Sites Using Peptide Arrays
ABOUT JoVE
Copyright © 2024 MyJoVE Corporation. All rights reserved