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Graz University of Technology

7 ARTICLES PUBLISHED IN JoVE

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Immunology and Infection

Microwave Assisted Rapid Diagnosis of Plant Virus Diseases by Transmission Electron Microscopy
Bernd Zechmann 1,2, Gerhard Graggaber 1, Günther Zellnig 1
1Institute of Plant Sciences, University of Graz, 2Institute for Electron Microscopy and Fine Structure Research, Graz University of Technology

This study describes a method that allows the rapid and clear diagnosis of plant virus diseases in about half a day by using a combination of microwave assisted plant sample preparation for transmission electron microscopy and negative staining methods.

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Engineering

The Preparation of Electrohydrodynamic Bridges from Polar Dielectric Liquids
Adam D. Wexler 1, Mónica López Sáenz 2, Oliver Schreer 2, Jakob Woisetschläger 3, Elmar C. Fuchs 1
1Applied Water Physics, Wetsus - Centre of Excellence for Sustainable Water Technology, 2IRCAM GmbH, 3Institute for Thermal Turbomachinery and Machine Dynamics, Graz University of Technology

Horizontal and vertical electrohydrodynamic liquid bridges are simple and powerful tools for exploring the interaction of high intensity electric fields and polar dielectric liquids. The construction of basic apparatus and operational examples, including thermographic images, for three liquids (e.g., water, DMSO, and glycerol) is presented.

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Biochemistry

In Situ Measurement and Correlation of Cell Density and Light Emission of Bioluminescent Bacteria
Eveline Brodl 1, Johannes Niederhauser 1, Peter Macheroux 1
1Institute of Biochemistry, Graz University of Technology

Bioluminescent bacteria regulate light production through a variety of mechanisms, such as quorum sensing. This novel method allows the in situ investigation of bioluminescence and the correlation of light emission to cell density. An artificial bioluminescent Escherichia coli system allows the characterization of lux operons, Lux proteins, and their interplay.

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Engineering

Measuring Sub-23 Nanometer Real Driving Particle Number Emissions Using the Portable DownToTen Sampling System
Markus Bainschab 1, Lukas Landl 2, Jon Andersson 3, Athanasios Mamakos 4, Stefan Hausberger 2, Alexander Bergmann 1
1Institute of Electrical Measurement and Sensor Systems, Graz University of Technology, 2Institute of Internal Combustion Engines and Thermodynamic, Graz University of Technology, 3Ricardo UK Ltd., 4AVL List GmbH

Presented here is the DownToTen (DTT) portable emission measurement system to assess real driving automotive emissions of sub-23 nm particles.

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Bioengineering

Affordable Oxygen Microscopy-Assisted Biofabrication of Multicellular Spheroids
Irina A. Okkelman *1, Chris Vercruysse *1, Alina V. Kondrashina 2, Sergey M. Borisov 3, Ruslan I. Dmitriev 1
1Tissue Engineering and Biomaterials Group, Department of Human Structure and Repair, Faculty of Medical and Health Sciences, Ghent University, 2Health and Happiness (H&H) Group, National Food Innovation Hub, 3Institute of Analytical Chemistry and Food Chemistry, Graz University of Technology

The protocol describes high-throughput spheroid generation for bioprinting using the multi-parametric analysis of their oxygenation and cell death on a standard fluorescence microscope. This approach can be applied to control the spheroids viability and perform standardization, which is important in modeling 3D tissue, tumor microenvironment, and successful (micro)tissue biofabrication.

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Engineering

Preparation of Free-Surface Hyperbolic Water Vortices
Roman Klymenko 1,5, Harmen Nanninga 2, Esther de Kroon 1, Luewton L. F. Agostinho 1,2, Elmar C. Fuchs 1,3, Jakob Woisetschläger 4, Wilfred F. L. M. Hoeben 5
1Wetsus - Centre of Excellence for Sustainable Water Technology, 2Water Technology Research Group, NHL Stenden University of Applied Sciences, 3Optical Sciences Group, Faculty of Science and Technology (TNW), University of Twente, 4Institute for Thermal Turbomachinery and Machine Dynamics, Graz University of Technology, 5Department of Electrical Engineering, Electrical Energy Systems group, Eindhoven University of Technology

This paper describes how three different water vortex regimes in a hyperbolic Schauberger funnel can be created, their most important characteristics, and how associated parameters such as the oxygen transfer rates can be calculated.

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Engineering

Additive Manufacturing-Enabled Low-Cost Particle Detector
Tanja Wallner 1,2, Markus Bainschab 1, Reinhard Klambauer 2, Alexander Bergmann 2
1Silicon Austria Labs, 2Graz University of Technology

Here, we present a protocol on how to build and test a simple but efficient low-cost particle detector.

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