Affordable Oxygen Microscopy-Assisted Biofabrication of Multicellular Spheroids

3.3K Views

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13:21 min

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April 6th, 2022

DOI :

10.3791/63403-v

April 6th, 2022

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Irina A. Okkelman*¹, Chris Vercruysse*¹, Alina V. Kondrashina², Sergey M. Borisov³, Ruslan I. Dmitriev¹

¹Tissue Engineering and Biomaterials Group, Department of Human Structure and Repair, Faculty of Medical and Health Sciences, Ghent University, ²Health and Happiness (H&H) Group, National Food Innovation Hub, ³Institute of Analytical Chemistry and Food Chemistry, Graz University of Technology

Summary

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Oxygen Microscopy

Biofabrication

Multicellular Spheroids

Fluorescence Microscopy

3D Printing

Vascularized Bone Tissue

Oxygen Probe

PDMS Stamps

Agarose Solution

Sterile Tissue Culture

Cell Culture Media

Dissociating Enzyme

Single Cell Suspension

Counting Chamber

Chapters in this video

0:04

Introduction

0:48

Preparing Micro-Patterned Agarose Coated Tissue Culture Plate

2:27

Preparing Oxygen Probe-Loaded Spheroids

3:50

Spheroids Bioprinting Procedure

5:48

Preparation of Spheroids for Live Imaging Analysis

7:10

Image Acquisition and Presentation

10:22

Results: Kinetic Study of Oxygen Probe and Comparative Analysis of Spheroids

12:18

Conclusion

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