The University of Texas Health Science Center

2 ARTICLES PUBLISHED IN JoVE

Behavior

A Community-based Stress Management Program: Using Wearable Devices to Assess Whole Body Physiological Responses in Non-laboratory Settings

Robert Carter III¹, Kirtigandha Salwe Carter², John Holliday³, Alice Holliday³, Carlton Keith Harrison⁴

¹Department of Emergency Medicine, The University of Texas Health Science Center, ²Department of Integrative Physiology, The University of North Texas Health Science Center, ³Works of Wonder International, ⁴DeVos Graduate Sports Business Management Program, University of Central Florida

Stress is an unavoidable and persistent component of life and holistic approaches for its management are being considered. A standardized methodology was created to demonstrate the feasibility of a breath-based stress management protocol that can be used with commercially available portable technology.

Cancer Research

Enhanced Viability for Ex vivo 3D Hydrogel Cultures of Patient-Derived Xenografts in a Perfused Microfluidic Platform

Lindsey K. Sablatura¹, Kristin M. Bircsak², Peter Shepherd³, Karla Queiroz⁴, Mary C. Farach-Carson^1,5,6, Pamela E. Constantinou^1,7, Anthony Saleh², Nora Navone³, Daniel A. Harrington^1,5,6

¹Department of BioSciences, Rice University, ²Mimetas US Inc, ³Department of Genitourinary Medical Oncology Research, Division of Cancer Medicine, The University of Texas MD Anderson Cancer Center, ⁴Mimetas B.V., ⁵Department of Bioengineering, Rice University, ⁶Department of Diagnostic and Biomedical Sciences, The University of Texas Health Science Center, ⁷Sheikh Ahmed Center for Pancreatic Cancer Research, The University of Texas MD Anderson Cancer Center

This protocol demonstrates methods to enable extended in vitro culture of patient-derived xenografts (PDX). One step enhances overall viability of multicellular cluster cultures in 3D hydrogels, through straightforward removal of non-viable single cells. A secondary step demonstrates best practices for PDX culture in a perfused microfluidic platform.