Enhanced Viability for Ex vivo 3D Hydrogel Cultures of Patient-Derived Xenografts in a Perfused Microfluidic Platform
6.0K Views
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12:12 min
•
December 5th, 2020
DOI :
December 5th, 2020
•Lindsey K. Sablatura1, Kristin M. Bircsak2, Peter Shepherd3, Karla Queiroz4, Mary C. Farach-Carson1,5,6, Pamela E. Constantinou1,7, Anthony Saleh2, Nora Navone3, Daniel A. Harrington1,5,6
1Department of BioSciences, Rice University, 2Mimetas US Inc, 3Department of Genitourinary Medical Oncology Research, Division of Cancer Medicine, The University of Texas MD Anderson Cancer Center, 4Mimetas B.V., 5Department of Bioengineering, Rice University, 6Department of Diagnostic and Biomedical Sciences, The University of Texas Health Science Center, 7Sheikh Ahmed Center for Pancreatic Cancer Research, The University of Texas MD Anderson Cancer Center
Chapters in this video
0:04
Introduction
1:09
PDX (Patient-Derived Xenografts) Dissociation and Initial Purification of Stromal Component
3:17
Density Gradient Centrifugation-Based Separation of PDX-Derived Clusters from Single Cells
6:03
Hydrogel Preparation and Microfluidic Plate Seeding
10:12
Results: Cell Staining and Viability Assessment
11:16
Conclusion
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