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Queensland University of Technology

12 ARTICLES PUBLISHED IN JoVE

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Neuroscience

Coherence between Brain Cortical Function and Neurocognitive Performance during Changed Gravity Conditions
Vera Brümmer 1, Stefan Schneider 1, Tobias Vogt 1, Heiko Strüder 1, Heather Carnahan 2, Christopher D. Askew 3, Roland Csuhaj 4
1Institute of Movement and Neurosciences, German Sport University Cologne, 2Deptartment of Surgical Skills, University of Toronto, 3School of Human Movement Studies, Institute of Health and Biomedical Innovation, Queensland University of Technology, 4Brain Products GmbH, Scientific Support, Gilching, Germany

The effect of weightlessness and hypergravity on both hemodynamic and electrophysiological processes in the brain is going to be followed during parabolic flight by EEG and NIRS techniques. A feasibility study of a more complex experiment, which is planned to carry out during medium- and long-term space flight.

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Medicine

Adjustable Stiffness, External Fixator for the Rat Femur Osteotomy and Segmental Bone Defect Models
Vaida Glatt 1, Romano Matthys 2
1Institute of Health and Biomedical Innovation, Queensland University of Technology, 2RISystem AG

One constraint of preclinical research in the field of bone repair is the lack of experimental control over the local mechanical environment within a healing bone lesion. We report the design and use of an external fixator for bone repair with the ability to change fixator stiffness in vivo.

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Developmental Biology

Isolation and Expansion of Mesenchymal Stem/Stromal Cells Derived from Human Placenta Tissue
Rebecca A. Pelekanos 1, Varda S. Sardesai 1, Kathryn Futrega 2, William B. Lott 2, Michael Kuhn 2, Michael R. Doran 2,3
1UQ Centre for Clinical Research, The University of Queensland, 2Translational Research Institute, Queensland University of Technology, 3Translational Research Institute, Mater Medical Research - University of Queensland

Herein we describe methods for the dissection of fetal and maternal tissues from human term placenta, followed by isolation and expansion of mesenchymal stem/stromal cells (MSC) from these tissues.

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Bioengineering

Melt Electrospinning Writing of Three-dimensional Poly(ε-caprolactone) Scaffolds with Controllable Morphologies for Tissue Engineering Applications
Felix M. Wunner 1, Onur Bas 1, Navid T. Saidy 1, Paul D. Dalton 2, Elena M. De-Juan Pardo 1, Dietmar W. Hutmacher 1,3,4
1ARC ITTC in Additive Biomanufacturing, Institute for Health and Biomedical Innovation (IHBI), Queensland University of Technology (QUT), 2Department for Functional Materials in Medicine and Dentistry and Bavarian Polymer Institute, University of Würzburg, 3Institute for Advanced Study, Technical University of Munich (TUM), 4George W Woodruff School of Mechanical Engineering, Georgia Institute of Technology

This protocol serves as a comprehensive guideline to fabricate scaffolds via electrospinning with polymer melts in a direct writing mode. We systematically outline the process and define the appropriate parameter settings for achieving targeted scaffold architectures.

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Chemistry

Measurements of Long-range Electronic Correlations During Femtosecond Diffraction Experiments Performed on Nanocrystals of Buckminsterfullerene
Rebecca A. Ryan 1, Sophie Williams 1, Andrew V. Martin 1, Ruben A. Dilanian 1, Connie Darmanin 2, Corey T. Putkunz 1, David Wood 3, Victor A. Streltsov 4, Michael W.M. Jones 5, Naylyn Gaffney 6, Felix Hofmann 7, Garth J. Williams 8, Sebastien Boutet 9, Marc Messerschmidt 10, M. Marvin Seibert 11, Evan K. Curwood 11, Eugeniu Balaur 2, Andrew G. Peele 5, Keith A. Nugent 2, Harry M. Quiney 1, Brian Abbey 2
1ARC Centre of Excellence in Advanced Molecular Imaging, School of Physics, University of Melbourne, 2Australian Research Council (ARC) Centre of Excellence in Advanced Molecular Imaging, Department of Chemistry and Physics, La Trobe Institute for Molecular Sciences, La Trobe University, 3Department of Physics, Imperial College London, 4Florey Institute of Neuroscience and Mental Health, 5Science and Engineering Faculty, Queensland University of Technology, 6Swinburne University of Technology, 7Department of Engineering Science, University of Oxford, 8Brookhaven National Laboratory, 9Linac Coherent Light Source, SLAC National Accelerator Laboratory, 10BioXFEL Science and Technology Center, 11Laboratory of Molecular Biophysics, Department of Cell and Molecular Biology, Uppsala University, 12Australian Synchrotron

We describe an experiment designed to probe the electronic damage induced in nanocrystals of Buckminsterfullerene (C60) by intense, femtosecond pulses of X-rays. The experiment found that, surprisingly, rather than being stochastic, the X-ray induced electron dynamics in C60 are highly correlated, extending over hundreds of unit cells within the crystals1.

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JoVE Journal

Optimization, Test and Diagnostics of Miniaturized Hall Thrusters
Jian W. M. Lim 1, Igor Levchenko 1,2, Muhammad W. A. B. Rohaizat 1, Shiyong Huang 1, Luxiang Xu 1, Yu Fei Sun 1, George C. Potrivitu 1, Jen S. Yee 1, Roysmond Z. W. Sim 1, Youmei Wang 3, Svitlana Levchenko 1, Kateryna Bazaka 2,1, Shuyan Xu 1
1Plasma Sources and Applications Centre, National Institute of Education, Nanyang Technological University, 2School of Chemistry, Physics, Mechanical Engineering, Queensland University of Technology, 3Department of Physics, School of Science, Hangzhou Dianzi University

Here, we present a protocol to test and optimize space propulsion systems based on miniaturized Hall-type thrusters.

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Medicine

Absorbent Microbiopsy Sampling and RNA Extraction for Minimally Invasive, Simultaneous Blood and Skin Analysis
Benson U.W. Lei 1,2, Miko Yamada 1, Van L.T. Hoang 3, Paul J. Belt 4, Mark H. Moore 5, Lynlee L. Lin 2, Ross Flewell-Smith 1, Nhung Dang 1,2, Shoko Tomihara 2, Tarl W. Prow 1,2
1Future Industries Institute, University of South Australia, 2Faculty of Medicine, University of Queensland, 3Faculty of Health, Queensland University of Technology, 4Department of Plastic and Reconstructive Surgery, Princess Alexandra Hospital, 5Australian Craniofacial Unit, Women's and Children's Hospital

In this article, we demonstrate how the absorbent microbiopsy technique is performed and how the sample can be used for RNA extraction for simple and simultaneous sampling of skin and blood in a minimally invasive manner.

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Bioengineering

Growth of Human and Sheep Corneal Endothelial Cell Layers on Biomaterial Membranes
Jennifer Walshe 1, Najla Al Khaled Abdulsalam 2, Shuko Suzuki 1, Traian V. Chirila 1,3,4,5,6,7,8, Damien G. Harkin 1,3,4
1Queensland Eye Institute, 2King Faisal University, 3School of Biomedical Sciences, Faculty of Health, Queensland University of Technology, 4Institute of Health and Biomedical Innovation, Queensland University of Technology, 5Science and Engineering Faculty, Queensland University of Technology, 6Faculty of Medicine, University of Queensland, 7Australian Institute for Bioengineering and Nanotechnology, University of Queensland, 8Faculty of Science, University of Western Australia

This protocol describes the critical steps required to establish and grow corneal endothelial cell cultures from explants of human or sheep tissue. A method for subculturing corneal endothelial cells on membranous biomaterials is also presented.

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Bioengineering

An Open Source Technology Platform to Manufacture Hydrogel-Based 3D Culture Models in an Automated and Standardized Fashion
Sebastian Eggert 1,2, Melanie Kahl 1,3, Ross Kent 1, Lukas Gaats 1, Nathalie Bock 1,3,4,5, Christoph Meinert 1,2, Dietmar W. Hutmacher 1,2,3,4,6
1Centre in Regenerative Medicine, Institute of Health and Biomedical Innovation, Queensland University of Technology, 2School of Mechanical, Medical and Process Engineering, Science and Engineering Faculty, Queensland University of Technology, 3School of Biomedical Sciences, Faculty of Health, Queensland University of Technology, 4Australian Prostate Cancer Research Centre, Institute of Health and Biomedical Innovation, Queensland University of Technology, 5Translational Research Institute, Queensland University of Technology, 6ARC ITTC in Additive Biomanufacturing, Institute of Health and Biomedical Innovation, Queensland University of Technology

This protocol serves as a comprehensive tutorial for standardized and reproducible mixing of viscous materials with a novel open source automation technology. Detailed instructions are provided on the operation of a newly developed open source workstation, the usage of an open source protocol designer, and the validation and verification to identify reproducible mixtures.

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Cancer Research

Rapid Optical Clearing for Semi-High-Throughput Analysis of Tumor Spheroids
Gency Gunasingh 1, Alexander P. Browning 2, Nikolas K. Haass 1
1The University of Queensland Diamantina Institute, The University of Queensland, 2School of Mathematical Sciences, Queensland University of Technology

Tumor spheroids are becoming increasingly utilized to assess tumor cell-microenvironment interactions and therapy response. The present protocol describes a robust but simple method for semi-high-throughput imaging of 3D tumor spheroids using rapid optical clearing.

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Engineering

Imaging of the Microstructural Failure Mechanism in the Human Hip
Saulo Martelli 1,2, Egon Perilli 2
1School of Mechanical, Medical and Process Engineering, Queensland University of Technology, 2Medical Device Research Institute, College of Science and Engineering, Flinders University

The protocol enables the measurement of the deformation of the bone microstructure in the entire proximal human femur and its toughness by combining large-volume micro-CT scanning, a custom-made compressive stage, and advanced image processing tools.

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Production and Optimization of LTE, a Leishmania tarentolae Derived Cell-Free Protein Expression System for Recombinant Protein Production
Wayne A. Johnston 1,2,3,4, Juan Alfaro Palma 1,2,3,4, Kirill Alexandrov 1,2,3,4,5
1CSIRO-QUT Synthetic Biology Alliance, Queensland University of Technology, 2ARC Centre of Excellence in Synthetic Biology, Queensland University of Technology, 3Centre for Agriculture and the Bioeconomy, Queensland University of Technology, 4School of Biology and Environmental Science, Queensland University of Technology, 5Centre for Genomics and Personalised Health, Queensland University of Technology

Leishmania Translational Extract (LTE) is a eukaryotic cell-free protein expression system derived from the single-celled parasite, Leishmania tarentolae. This optimized protocol makes LTE simple and cost-effective to manufacture. It is suitable for various applications focused on the multiparallel expression and study of complex eukaryotic proteins and their interactions.

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