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University of Freiburg

4 ARTICLES PUBLISHED IN JoVE

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Immunology and Infection

Granulocyte-dependent Autoantibody-induced Skin Blistering
Kinga Csorba 1, Sebastian Sitaru 2, Cassian Sitaru 1,3
1Department of Dermatology, University of Freiburg , 2Kepler High School Freiburg, 3Centre for Biological Signalling Studies (BIOSS), University of Freiburg

In the animal model described in our present work, purified IgG antibodies against a stretch of 200 amino acids (aa 757-967) of collagen VII are injected repeatedly into mice reproducing the blistering phenotype as well as the histo- and immunopathological features characteristic to human epidermolysis bullosa acquisita (EBA)1.

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Bioengineering

Time-lapse Fluorescence Imaging of Arabidopsis Root Growth with Rapid Manipulation of The Root Environment Using The RootChip
Guido Grossmann 1, Matthias Meier 2,3,4, Heather N. Cartwright 1, Davide Sosso 1, Stephen R. Quake 2,3, David W. Ehrhardt 1, Wolf B. Frommer 1
1Department of Plant Biology, Carnegie Institution for Science, 2Howard Hughes Medical Institute, 3Departments of Applied Physics and Bioengineering, Stanford University , 4Department of Microsystems Engineering (IMTEK) and Center for Biological Signaling Studies (BIOSS), University of Freiburg

This article provides a protocol for cultivation of Arabidopsis seedlings in the RootChip, a microfluidic imaging platform that combines automated control of growth conditions with microscopic root monitoring and FRET-based measurement of intracellular metabolite levels.

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Neuroscience

Analyzing Murine Schwann Cell Development Along Growing Axons
Stephan Heermann 1,2, Kerstin Krieglstein 1,3
1Department of Molecular Embryology, Institute of Anatomy and Cell Biology, University of Freiburg , 2Department of Neuroanatomy, University of Heidelberg, 3FRIAS, University of Freiburg

Here we describe a Schwann cell (SC) migration assay in which SCs are able to develop along extending axons.

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Neuroscience

Isolation and Culture of Mouse Cortical Astrocytes
Sebastian Schildge 1, Christian Bohrer 1, Kristina Beck 2, Christian Schachtrup 1
1Institute of Anatomy and Cell Biology, University of Freiburg , 2Centre of Chronic Immunodeficiency (CCI), University Medical Centre Freiburg, University of Freiburg

Astrocytes have been recognized to be versatile cells participating in fundamental biological processes that are essential for normal brain development and function, and central nervous system repair. Here we present a rapid procedure to obtain pure mouse astrocyte cultures to study the biology of this major class of central nervous system cells.

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