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Isolation and Culture of Mouse Cortical Astrocytes

DOI :

10.3791/50079-v

January 19th, 2013

January 19th, 2013

87,396 Views

1Institute of Anatomy and Cell Biology, University of Freiburg , 2Centre of Chronic Immunodeficiency (CCI), University Medical Centre Freiburg, University of Freiburg

Astrocytes have been recognized to be versatile cells participating in fundamental biological processes that are essential for normal brain development and function, and central nervous system repair. Here we present a rapid procedure to obtain pure mouse astrocyte cultures to study the biology of this major class of central nervous system cells.

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