University of Freiburg

4 ARTICLES PUBLISHED IN JoVE

Immunology and Infection

Granulocyte-dependent Autoantibody-induced Skin Blistering

Kinga Csorba¹, Sebastian Sitaru², Cassian Sitaru^1,3

¹Department of Dermatology, University of Freiburg , ²Kepler High School Freiburg, ³Centre for Biological Signalling Studies (BIOSS), University of Freiburg

In the animal model described in our present work, purified IgG antibodies against a stretch of 200 amino acids (aa 757-967) of collagen VII are injected repeatedly into mice reproducing the blistering phenotype as well as the histo- and immunopathological features characteristic to human epidermolysis bullosa acquisita (EBA)¹.

Bioengineering

Time-lapse Fluorescence Imaging of Arabidopsis Root Growth with Rapid Manipulation of The Root Environment Using The RootChip

Guido Grossmann¹, Matthias Meier^2,3,4, Heather N. Cartwright¹, Davide Sosso¹, Stephen R. Quake^2,3, David W. Ehrhardt¹, Wolf B. Frommer¹

¹Department of Plant Biology, Carnegie Institution for Science, ²Howard Hughes Medical Institute, ³Departments of Applied Physics and Bioengineering, Stanford University , ⁴Department of Microsystems Engineering (IMTEK) and Center for Biological Signaling Studies (BIOSS), University of Freiburg

This article provides a protocol for cultivation of Arabidopsis seedlings in the RootChip, a microfluidic imaging platform that combines automated control of growth conditions with microscopic root monitoring and FRET-based measurement of intracellular metabolite levels.

Neuroscience

Analyzing Murine Schwann Cell Development Along Growing Axons

Stephan Heermann^1,2, Kerstin Krieglstein^1,3

¹Department of Molecular Embryology, Institute of Anatomy and Cell Biology, University of Freiburg , ²Department of Neuroanatomy, University of Heidelberg, ³FRIAS, University of Freiburg

Here we describe a Schwann cell (SC) migration assay in which SCs are able to develop along extending axons.

Neuroscience

Isolation and Culture of Mouse Cortical Astrocytes

Sebastian Schildge¹, Christian Bohrer¹, Kristina Beck², Christian Schachtrup¹

¹Institute of Anatomy and Cell Biology, University of Freiburg , ²Centre of Chronic Immunodeficiency (CCI), University Medical Centre Freiburg, University of Freiburg

Astrocytes have been recognized to be versatile cells participating in fundamental biological processes that are essential for normal brain development and function, and central nervous system repair. Here we present a rapid procedure to obtain pure mouse astrocyte cultures to study the biology of this major class of central nervous system cells.