JoVE Logo

Sign In

University of Turku

6 ARTICLES PUBLISHED IN JoVE

image

Developmental Biology

Analysis of Epididymal Protein Synthesis and Secretion
Wei Zhou 1,2, Petra Sipilä 3, Geoffry N. De Iuliis 1,2, Matthew D. Dun 2,4, Brett Nixon 1,2
1Priority Research Centre for Reproductive Science, School of Environmental and Life Sciences, University of Newcastle, 2Hunter Medical Research Institute, 3Department of Physiology, Turku Center for Disease Modeling, Institute of Biomedicine, University of Turku, 4School of Biomedical Sciences and Pharmacy, Faculty of Health and Medicine, University of Newcastle

Here, we report the immunofluorescence localization of dynamin to illustrate the protocols for the detection of proteins in paraffin-embedded mouse epididymal sections and those of an immortalized epididymal cell line (mECap18). We also describe the protocols for the isolation of secretory proteins from both epididymal fluid and conditioned cell media.

image

Biochemistry

Analysis of Thylakoid Membrane Protein Complexes by Blue Native Gel Electrophoresis
Marjaana Rantala 1, Virpi Paakkarinen 1, Eva-Mari Aro 1
1Molecular Plant Biology, Department of Biochemistry, University of Turku

A protocol for the elucidation of plant thylakoid protein complex organization and composition with blue native polyacrylamide gel electrophoresis (BN-PAGE) and 2D-SDS-PAGE is described. The protocol is optimized for Arabidopsis thaliana, but can be used for other plant species with minor modifications.

image

Developmental Biology

Enrichment of Pachytene Spermatocytes and Spermatids from Mouse Testes Using Standard Laboratory Equipment
Matteo Da Ros *1, Tiina Lehtiniemi *1, Opeyemi Olotu 1, Oliver Meikar 1, Noora Kotaja 1
1Institute of Biomedicine, University of Turku

Presented here is a protocol for enriching pachytene spermatocytes, round spermatids, and elongating spermatids from adult mouse testes using a discontinuous bovine serum albumin density gradient with standard laboratory equipment.

image

Biochemistry

Deciphering the Structural Effects of Activating EGFR Somatic Mutations with Molecular Dynamics Simulation
Mahlet Z. Tamirat 1, Kari J. Kurppa 2, Klaus Elenius 2,3,4, Mark S. Johnson 1
1Structural Bioinformatics Laboratory, Biochemistry, Faculty of Science and Engineering, Åbo Akademi University, 2Medicity Research Laboratories and Institute of Biomedicine, University of Turku, 3Turku Bioscience Centre, University of Turku and Åbo Akademi University, 4Department of Oncology and Radiotherapy, University of Turku and Turku University Hospital

The objective of this protocol is to use molecular dynamics simulations to examine the dynamic structural changes that occur due to activating mutations of the EGFR kinase protein.

image

Developmental Biology

Transillumination-Assisted Dissection of Specific Stages of the Mouse Seminiferous Epithelial Cycle for Downstream Immunostaining Analyses
Juho-Antti Mäkelä 1, Sheyla Cisneros-Montalvo 1, Tiina Lehtiniemi 1, Opeyemi Olotu 1, Hue M. La 3,4, Jorma Toppari 1,2, Robin M. Hobbs 3,4, Martti Parvinen 1, Noora Kotaja 1
1Institute of Biomedicine, Integrative Physiology and Pharmacology Unit, University of Turku, Turku, Finland, 2Department of Pediatrics, Turku University Hospital, Turku, Finland, 3Centre for Reproductive Health, Hudson Institute of Medical Research, Melbourne, VIC 3168, Australia, 4Australian Regenerative Medicine Institute, Monash University, Melbourne, VIC 3800, Australia

This protocol describes transillumination-assisted microdissection of segments of adult mouse seminiferous tubules representing specific stages of seminiferous epithelial cycle, and cell types therein, and subsequent immunostaining of squash preparations and intact tubule segments.

image

Environment

Quantification of the Potential Impact of Glyphosate-Based Products on Microbiomes
Suni Anie Mathew 1, Anne Muola 1, Kari Saikkonen 1, Irma Saloniemi 2, Marjo Helander 2, Pere Puigbò 2,3,4
1Biodiversity Unit, University of Turku, 2Department of Biology, University of Turku, 3Nutrition and Health Unit, Eurecat Technology Centre of Catalonia, 4Department of Biochemistry and Biotechnology, Rovira i Virgili University

Glyphosate-based products (GBP) are the most common broad-spectrum herbicides worldwide. In this article, we introduce general guidelines to quantify the effect of GBP on microbiomes, from field experiments to bioinformatics analyses.

JoVE Logo

Privacy

Terms of Use

Policies

Research

Education

ABOUT JoVE

Copyright © 2024 MyJoVE Corporation. All rights reserved