Transillumination-Assisted Dissection of Specific Stages of the Mouse Seminiferous Epithelial Cycle for Downstream Immunostaining Analyses

6.9K Views

09:59 min

October 7th, 2020

DOI :

10.3791/61800-v

October 7th, 2020


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Transillumination assisted Dissection

Chapters in this video

0:04

Introduction

0:51

Testis Dissection and Preparation

2:10

Transillumination-Assisted Microdissection

2:47

Squash Preparation and Stage Verification

4:04

Whole-Mount Seminiferous Tubule Immunostaining

7:04

Results: Representative Mouse Seminiferous Epithelial Cycle Staging

9:16

Conclusion

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