Transillumination-Assisted Dissection of Specific Stages of the Mouse Seminiferous Epithelial Cycle for Downstream Immunostaining Analyses

7.0K Views

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09:59 min

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October 7th, 2020

DOI :

10.3791/61800-v

October 7th, 2020

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Juho-Antti Mäkelä¹, Sheyla Cisneros-Montalvo¹, Tiina Lehtiniemi¹, Opeyemi Olotu¹, Hue M. La³^,⁴, Jorma Toppari¹^,², Robin M. Hobbs³^,⁴, Martti Parvinen¹, Noora Kotaja¹

¹Institute of Biomedicine, Integrative Physiology and Pharmacology Unit, University of Turku, Turku, Finland, ²Department of Pediatrics, Turku University Hospital, Turku, Finland, ³Centre for Reproductive Health, Hudson Institute of Medical Research, Melbourne, VIC 3168, Australia, ⁴Australian Regenerative Medicine Institute, Monash University, Melbourne, VIC 3800, Australia

Summary

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Transillumination assisted Dissection

Seminiferous Epithelial Cycle

Spermatogenesis

Stage specific Tubules

Immunostaining Analysis

Adult Male Mouse

Tunica Albuginea

Microdissection Scissors

Phase contrast Microscope

PBS phosphate buffered Saline

Dissecting Microscope

Cell Monolayer

Opeyemi Olotu

Dissection Protocol

Chapters in this video

0:04

Introduction

0:51

Testis Dissection and Preparation

2:10

Transillumination-Assisted Microdissection

2:47

Squash Preparation and Stage Verification

4:04

Whole-Mount Seminiferous Tubule Immunostaining

7:04

Results: Representative Mouse Seminiferous Epithelial Cycle Staging

9:16

Conclusion

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