Analysis of Epididymal Protein Synthesis and Secretion

9.3K Views

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10:23 min

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August 25th, 2018

DOI :

10.3791/58308-v

August 25th, 2018

•

Wei Zhou¹^,², Petra Sipilä³, Geoffry N. De Iuliis¹^,², Matthew D. Dun²^,⁴, Brett Nixon¹^,²

¹Priority Research Centre for Reproductive Science, School of Environmental and Life Sciences, University of Newcastle, ²Hunter Medical Research Institute, ³Department of Physiology, Turku Center for Disease Modeling, Institute of Biomedicine, University of Turku, ⁴School of Biomedical Sciences and Pharmacy, Faculty of Health and Medicine, University of Newcastle

Summary

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Keywords Epididymal Protein Synthesis

Chapters in this video

0:04

Title

0:36

Immunofluorescence Staining of the Paraffin-embedded Epididymal Sections

2:44

Isolation of Epididymosomes from the Mouse Caput Epididymis

5:26

Immunofluorescence Staining of mECap18 Cells

6:22

Isolation of Proteins from Conditioned Cell Culture Medium

8:06

Results: Analysis of Epididymal Protein Synthesis and Secretion

9:45

Conclusion

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