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Semmelweis University

15 ARTICLES PUBLISHED IN JoVE

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Medicine

Stem Cell Transplantation in an in vitro Simulated Ischemia/Reperfusion Model
Attila Cselenyák 1, Zsolt Benko 1, Mónika Szepes 1, Levente Kiss 1, Zsombor Lacza 1
1Institute of Human Physiology and Clinical Experimental Research, Semmelweis University

We demonstrate how to set up an in vitro ischemia/reperfusion model and how to evaluate the effect of stem cell therapy on postischemic cardiac cells.

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Cancer Research

In Vitro Imaging and Quantification of the Drug Targeting Efficiency of Fluorescently Labeled GnRH Analogues
József Murányi 1,2, Attila Varga 1, Bianka Gurbi 1, Pál Gyulavári 1, Gábor Mező 3, Tibor Vántus 2
1MTA-SE Pathobiochemistry Research Group, Semmelweis University, 2Department of Medical Chemistry, Molecular Biology and Pathobiochemistry, Semmelweis University, 3MTA-ELTE Research Group of Peptide Chemistry, Eötvös Loránd University

Selectively labeled fluorescent GnRH-I, -II and -III derivatives are reliable tools for tracking and quantifying their cellular uptake. This manuscript introduces experiments to visualize, quantify and compare the uptake efficiency of these GnRH conjugates in various cell lines.

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Biology

Isolation of Retinal Arterioles for Ex Vivo Cell Physiology Studies
Tim M. Curtis *1, Declan McLaughlin 2, Michael O'Hare 1, Joanna Kur 1, Peter Barabas 1, Gordon Revolta 1, C. Norman Scholfield 3, J. Graham McGeown 4, Mary K. McGahon *2
1Centre for Experimental Medicine, Queen's University of Belfast, 2Centre for Biomedical Sciences (Education), Queen's University of Belfast, 3Department of Pharmaceutical Chemistry and Pharmacognosy, Naresuan University, 4School of Medicine, Dentistry and Biomedical Sciences, Queen's University of Belfast

This manuscript describes a straightforward protocol for the isolation of arterioles from the rat retina that can be used in electrophysiological, calcium imaging and pressure myography studies.

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Medicine

A Novel Approach to Monitoring Graft Neovascularization in the Human Gingiva
Reka Fazekas 1, Eszter Molnár 1, Barbara Mikecs 1, Zsolt Lohinai 1, János Vág 1
1Department of Conservative Dentistry, Faculty of Dentistry, Semmelweis University

This study introduces a protocol for measuring microcirculation in human oral mucosa by laser speckle contrast imaging. The monitoring of wound healing after vestibuloplasty combined with a xenogenic collagen graft is presented on a clinical case.

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Medicine

Detection of Vascular Pathways of Oral Mucosa Influencing Soft- and Hard Tissue Surgeries by Latex Milk Injection
Arvin Shahbazi 1,2, Ulrike Pilsl 3, Bálint Molnár 2, Georg Feigl 3
1Department of Anatomy, Histology and Embryology, Semmelweis University, 2Department of Periodontology, Semmelweis University, 3Department of Macroscopical and Clinical Anatomy, Medical University of Graz

Here, we describe the staining of blood vessels using latex milk injections. This procedure provides anatomical knowledge of the oral blood supply for detailed macroscopic vascular mapping of the oral mucosa, as well as understanding of proper flap design to prevent complications and promote postoperative wound healing.

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Bioengineering

Evaluation of Left Ventricular Structure and Function using 3D Echocardiography
Adrienn Ujvári 1, Bálint Károly Lakatos 1, Márton Tokodi 1, Alexandra Fábián 1, Béla Merkely 1, Attila Kovács 1
1Heart and Vascular Center, Semmelweis University

In this article, we provide a step-by-step acquisition and analysis protocol for the volumetric assessment and speckle-tracking analysis of the left ventricle by 3D echocardiography, particularly focusing on practical aspects that maximize the feasibility of this technique.

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Medicine

Morphological and Functional Assessment of the Right Ventricle Using 3D Echocardiography
Bálint Károly Lakatos 1, Márton Tokodi 1, Erika Kispál 1, Béla Merkely 1, Attila Kovács 1
1Semmelweis University Heart and Vascular Center

Here, we provide a step-by-step acquisition and analysis protocol for the 3D volumetric assessment of the right ventricle, mainly focusing on the practical aspects that maximize the feasibility of this technique.

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Medicine

Echocardiographic Evaluation of Atrial Communications before Transcatheter Closure
Andrea Ágnes Molnár *1, Pál Ábrahám *1, Béla Merkely 1, Sándor Nardai 1
1Heart and Vascular Center, Semmelweis University

Transthoracic (TTE) and transesophageal (TEE) echocardiography represent the basic imaging tools for interatrial septum examination. Three dimensional (3D) TEE provides incremental information in the assessment of the interatrial septum. Further advanced echocardiography techniques using speckle tracking echocardiography are applied for sensitive volumetric and functional assessment of the heart chambers. 

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Examination of Propylene Carbonate as a Sustainable Green Solvent for Suzuki Cross-Coupling Reactions under Microwave and Conventional Circumstances
Balázs László Pásztor 1, Andrea Czompa 1, Jennifer Sahar Alizadeh 1, István M. Mándity 1,2
1Department of Organic Chemistry, Faculty of Pharmacy, Semmelweis University, 2MTA TTK Lendulet Artificial Transporter Research Group, Institute of Materials and Environmental Chemistry, Research Center for Natural Sciences, Hungarian Academy of Sciences

Propylene carbonate has been shown to be a prominent green solvent in Suzuki cross-coupling reactions. Furthermore, the microwave reactor is demonstrated to afford enhanced reaction yields with decreasing reaction times. Using either a microwave reactor or conventional heating, propylene carbonate is sustainable for the cross-coupling reaction.

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Medicine

Post-Myocardial Infarction Heart Failure in Closed-chest Coronary Occlusion/Reperfusion Model in Göttingen Minipigs and Landrace Pigs
Gábor B Brenner *1,2, Zoltán Giricz *1,2, Rita Garamvölgyi 3,4, András Makkos 1, Zsófia Onódi 1, Nabil V Sayour 1, Tamás G Gergely 1, Tamás Baranyai 1, Örs Petneházy 3,4, Dénes Kőrösi 3, Gergő P. Szabó 3, Hajnalka Vago 5, Zsófia Dohy 5, Csilla Czimbalmos 5, Béla Merkely 5, Swetlana Boldin-Adamsky 6, Elena Feinstein 6, Iván G Horváth 7, Péter Ferdinandy 1,2
1Department of Pharmacology and Pharmacotherapy, Semmelweis University, 2Pharmahungary Group, 3Diagnostic, Radiation Oncology, Research and Teaching Center, Moritz Kaposi Somogy County Teaching Hospital, 4Faculty of Agricultural and Environmental Sciences, Kaposvar University, 5Heart and Vascular Center, Semmelweis University, 6Quark Pharmaceuticals, Inc, 7Heart Institute, Medical School, University of Pécs

The overall goal of the current study is to present the techniques of induction of myocardial infarction (MI) and post-myocardial infarction heart failure (post-MI HF) in closed-chest, adult Göttingen minipigs and the characterization of post-MI HF model in Göttingen minipigs as compared to Landrace pigs.

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Medicine

Time-Resolved, Dynamic Computed Tomography Angiography for Characterization of Aortic Endoleaks and Treatment Guidance via 2D-3D Fusion-Imaging
Marton Berczeli 1,2, Ponraj Chinnadurai 1,3, Su Min Chang 4, Alan B. Lumsden 1
1Department of Cardiovascular Surgery, Houston Methodist Hospital, 2Department of Vascular and Endovascular Surgery, Semmelweis University, 3Advanced Therapies, Siemens Medical Solutions USA Inc., 4Department of Cardiology, Houston Methodist Hospital

Dynamic computed tomography angiography (CTA) imaging provides additional diagnostic value in characterizing aortic endoleaks. This protocol describes a qualitative and quantitative approach using time-attenuation curve analysis to characterize endoleaks. The technique of integrating dynamic CTA imaging with fluoroscopy using 2D-3D image fusion is illustrated for better image guidance during treatment.

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Medicine

Dynamic Navigation for Dental Implant Placement
Gabor Tamas Pinter 1, Roland Decker 1, Gabor Szenasi 2, Peter Barabas 1, Tamas Huszar 1
1Department of Oro-Maxillofacial Surgery and Stomatology, Faculty of Dentistry, Semmelweis University, 2Doctoral School Of Theoretical And Translational Medicine, Schools of PhD Studies, Semmelweis University

Dynamic computer-aided implant surgery (DCAIS) is a controlled implant surgical placement method performed without a surgical template using optical control. The real-time intraoperative control of movement and position of the surgical device simplifies the procedure and gives more freedom to the surgeon, providing similar precision as static navigation methods.

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Neuroscience

A Minimally Invasive, Fast Spinal Cord Lateral Hemisection Technique for Modeling Open Spinal Cord Injuries in Rats
Krisztián B. Csomó 1, Gábor Varga 2,3, Andrea A. Belik 4, László Hricisák 5, Zoltán Borbély 2, Gábor Gerber 6
1Department of Conservative Dentistry, Faculty of Dentistry, Semmelweis University, 2Department of Oral Biology, Faculty of Dentistry, Semmelweis University, 3Centre for Translational Medicine, Semmelweis University, 4Department of Molecular Biology, Faculty of Medicine, Semmelweis University, 5Institute of Translational Medicine, Faculty of Medicine, Semmelweis University, 6Department of Anatomy, Histology and Embryology, Faculty of Medicine, Semmelweis University

Here, we describe a new, fast technique modeling open spinal cord injury in rats that eliminates laminectomy. Lateral hemisection is performed while viewing through a microscope. The technique is versatile and can also be used in the cervical, thoracic, and lumbar regions of the spinal cord of other animals.

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3D Echocardiography: Toward a Better Understanding of Cardiac Anatomy and Function
Attila Kovács 1
1Heart and Vascular Center, Semmelweis University

3D Echocardiography: Toward a Better Understanding of Cardiac Anatomy and Function

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Neuroscience

In vivo Characterization of Endocrine Disrupting Chemical Effects via Thyroid Hormone Action Indicator Mouse
Richárd Sinkó 1,2, Petra Mohácsik 1, Csaba Fekete *3, Balázs Gereben *1
1Laboratory of Molecular Cell Metabolism, Institute of Experimental Medicine, 2János Szentágothai PhD School of Neurosciences, Semmelweis University, 3Laboratory of Integrative Neuroendocrinology, Institute of Experimental Medicine

The Thyroid Hormone Action Indicator mouse model was developed to enable tissue-specific quantification of local thyroid hormone action using its endogenous regulatory machinery. Recently, it has been shown that the model is suitable for characterizing endocrine-disrupting chemicals interacting with thyroid hormone economy, both by ex vivo and in vivo methodologies.

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