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14.0K Views
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09:15 min
November 5th, 2011
DOI :
10.3791/3575-v
Chapters
0:05
Title
2:20
Preparing H9c2 Cardiomyoblast Cells
3:44
Simulated Ischemia/Reoxygenation
5:25
Preparing Rescue Mesenchymal Stem Cells
6:21
Live/Dead Staining of Co-cultivated Cells
7:23
Analysis with Confocal Microscopy
7:47
Analysis with Flow Cytometry
8:36
Representative Results
Summary
We demonstrate how to set up an in vitro ischemia/reperfusion model and how to evaluate the effect of stem cell therapy on postischemic cardiac cells.
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