In Vitro Imaging and Quantification of the Drug Targeting Efficiency of Fluorescently Labeled GnRH Analogues

7.5K Views

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10:36 min

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March 21st, 2017

DOI :

10.3791/55529-v

March 21st, 2017

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József Murányi¹^,², Attila Varga¹, Bianka Gurbi¹, Pál Gyulavári¹, Gábor Mező³, Tibor Vántus²

¹MTA-SE Pathobiochemistry Research Group, Semmelweis University, ²Department of Medical Chemistry, Molecular Biology and Pathobiochemistry, Semmelweis University, ³MTA-ELTE Research Group of Peptide Chemistry, Eötvös Loránd University

Summary

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Keywords In Vitro Imaging

Quantification

Drug Targeting Efficiency

Fluorescently Labeled GnRH Analogues

Human Cancer Cell Cultures

Targeted Anti cancer Therapy

Preheated Treating Media

Fixing Solution

Fluorescent Probe

Mounting Medium

Chapters in this video

0:05

Title

1:07

GnRH Uptake

2:39

Type I GnRH Receptor Expression

5:39

Fluorescence Microscopy Imaging

6:26

FACS-assisted GnRH Quantification

8:00

Results: GnRH I Uptake and Type I GnRH Receptor Expression in Pharynx Carcinoma Cells

9:25

Conclusion

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