JoVE Logo

Sign In

Ragon Institute of MGH, MIT, and Harvard

2 ARTICLES PUBLISHED IN JoVE

image

Immunology and Infection

Determining the Phagocytic Activity of Clinical Antibody Samples
Elizabeth G. McAndrew 1, Anne-Sophie Dugast 1, Anna F. Licht 1, Justin R. Eusebio 1, Galit Alter 1, Margaret E. Ackerman 2
1Massachusetts General Hospital, Ragon Institute of MGH, MIT, and Harvard, 2Thayer School of Engineering, Dartmouth College

We present a high-throughput flow cytometric assay to determine the phagocytic activity of antigen-specific antibodies from clinical samples, utilizing fluorescent antigen-coated beads and a monocytic cell line expressing multiple Fc receptors—providing receptor usage and phagocytic activity determinations in a standardized and reproducible fashion for any antigen of interest.

image

Immunology and Infection

New Tools to Expand Regulatory T Cells from HIV-1-infected Individuals
Mathieu Angin 1, Melanie King 1, Marylyn Martina Addo 1,2
1Ragon Institute of MGH, MIT, and Harvard, 2Division of Infectious Diseases, Massachusetts General Hospital

CD4+ Regulatory T cells are potent immune-modulators and serve important functions in immune homeostasis. The paucity of these cells in peripheral blood makes functional studies challenging, specifically in the context of HIV-1-infection. We here describe a method to isolate and expand functional CD4+ Tregs from peripheral blood from HIV-1-infected individuals.

JoVE Logo

Privacy

Terms of Use

Policies

Research

Education

ABOUT JoVE

Copyright © 2024 MyJoVE Corporation. All rights reserved