JoVE Logo
Faculty Resource Center

Sign In

Determining the Phagocytic Activity of Clinical Antibody Samples

DOI :

10.3791/3588-v

9:05 min

November 30th, 2011

November 30th, 2011

22,946 Views

1Massachusetts General Hospital, Ragon Institute of MGH, MIT, and Harvard, 2Thayer School of Engineering, Dartmouth College

We present a high-throughput flow cytometric assay to determine the phagocytic activity of antigen-specific antibodies from clinical samples, utilizing fluorescent antigen-coated beads and a monocytic cell line expressing multiple Fc receptors—providing receptor usage and phagocytic activity determinations in a standardized and reproducible fashion for any antigen of interest.

Tags

Phagocytic Activity

-- Views

Related Videos

article

Determining the Reactivity and Titre of Serum using a Haemagglutination Assay

article

Using a Pan-Viral Microarray Assay (Virochip) to Screen Clinical Samples for Viral Pathogens

article

Multiplex Detection of Bacteria in Complex Clinical and Environmental Samples using Oligonucleotide-coupled Fluorescent Microspheres

article

Determining Optimal Cytotoxic Activity of Human Her2neu Specific CD8 T cells by Comparing the Cr51 Release Assay to the xCELLigence System

article

Saliva, Salivary Gland, and Hemolymph Collection from Ixodes scapularis Ticks

article

Fluorescence Microscopy Methods for Determining the Viability of Bacteria in Association with Mammalian Cells

article

Scalable High Throughput Selection From Phage-displayed Synthetic Antibody Libraries

article

A Rapid Strategy for the Isolation of New Faustoviruses from Environmental Samples Using Vermamoeba vermiformis

article

Fast and Specific Assessment of the Halogenating Peroxidase Activity in Leukocyte-enriched Blood Samples

article

Assessing Retinal Microglial Phagocytic Function In Vivo Using a Flow Cytometry-based Assay

JoVE Logo

Privacy

Terms of Use

Policies

Research

Education

ABOUT JoVE

Copyright © 2024 MyJoVE Corporation. All rights reserved