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huaqiao university

5 ARTICLES PUBLISHED IN JoVE

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Biology

In situ Hybridization for Sipunculus nudus Coelomic Fluid
Wenhua Li 1, Mingrui Yuan 1, Yaqin Wu 1
1Key Laboratory of Xiamen Marine and Gene Drugs, School of Biomedical Sciences, Huaqiao University

This protocol describes an effective in situ hybridization approach to detect the mRNA expression levels and spatial patterns of target genes in Sipunculus nudus coelomic fluid.

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Bioengineering

Fabricating Highly Open Porous Microspheres (HOPMs) via Microfluidic Technology
Sheng-Chang Luo 1,2, Ying Wang 3, Ranjith Kumar Kankala 1,2, Yu Shrike Zhang 4, Ai-Zheng Chen 1,2
1Institute of Biomaterials and Tissue Engineering, Huaqiao University, 2Fujian Provincial Key Laboratory of Biochemical Technology, Huaqiao University, 3Affiliated Dongguan Hospital, Southern Medical University, 4Division of Engineering in Medicine, Department of Medicine, Brigham and Women's Hospital, Harvard Medical School

The present protocol describes the fabrication of poly(lactic-co-glycolic acid)-based highly open porous microspheres (HOPMs) via the single-emulsion formulation based facile microfluidic technology. These microspheres have potential applications in tissue engineering and drug screening.

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Biochemistry

Affinity Purification of a Fibrinolytic Enzyme from Sipunculus nudus
Mingqing Tang 1,2, Hongjun Lin 1, Chengjia Hu 1, Hui Yan 1,2
1Medicine School, Huaqiao University, 2Engineering Research Centre of Molecular Medicine of Ministry of Education

Here, we present an affinity purification method of a fibrinolytic enzyme from Sipunculus nudus that is simple, inexpensive, and efficient.

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Biochemistry

A Comprehensive Approach to Analyze the Cell Components of Cerebral Blood Clots
Wenwu Lin *1, Hongjun Lin *2, Pengliang Xin 3, Hui Yan 2, Bin Kang 4, Mingqing Tang 2
1Emergency Department, Quanzhou First Hospital Affiliated to Fujian Medical University, 2Medicine School, Huaqiao University, 3Department of Haematology, Quanzhou First Hospital Affiliated to Fujian Medical University, 4Department of Laboratory Medicine, Quanzhou First Hospital Affiliated to Fujian Medical University

This study describes a fast and effective method for the cell component analysis of cerebral blood clots through clot dissolving, cell staining, and routine blood examination.

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Biochemistry

Rapid Separation and Display of Active Fibrinogenolytic Agents in Sipunculus nudus through Fibrinogen-Polyacrylamide Gel Electrophoresis
Bin Kang *1, Chengjia Hu *2, Hongjun Lin 2, Hui Yan 2, Chengyun Wei 3, Mingqing Tang 2
1Department of Laboratory Medicine, Quanzhou First Hospital Affiliated to Fujian Medical University, 2Medicine School, Huaqiao University, 3Department of Laboratory Medicine, Hospital of Huaqiao University

Here, we present a fibrinogen-polyacrylamide gel electrophoresis (PAGE) protocol to rapidly separate and display the fibrinogenolytic agents of Sipunculus nudus.

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