Academic Medical Center Amsterdam

2 ARTICLES PUBLISHED IN JoVE

Medicine

The Use of Pharmacological-challenge fMRI in Pre-clinical Research: Application to the 5-HT System

Anne Klomp¹, Jordi L. Tremoleda², Anouk Schrantee¹, Willy Gsell², Liesbeth Reneman¹

¹Department of Radiology, Brain Imaging Center, Academic Medical Center Amsterdam, ²Biological Imaging Centre, MRC Clinical Sciences Centre, Imperial College London

The goal of this technique is to assess serotonin (5-HT) neurotransmitter function in the live and free-breathing animal with pharmacological magnetic resonance imaging (phMRI) and an intravenous challenge with a selective serotonin reuptake inhibitor (SSRI), fluoxetine.

Developmental Biology

A Submerged Filter Paper Sandwich for Long-term Ex Ovo Time-lapse Imaging of Early Chick Embryos

Manuel Schmitz^*1, Ben K. A. Nelemans^*1, Theodoor H. Smit²

¹Department of Orthopaedic Surgery, VU University Medical Center Amsterdam, MOVE Research Institute Amsterdam, ²Department of Anatomy, Embryology & Physiology, Academic Medical Center Amsterdam

Here, we present a new technique to culture chick embryos ex ovo for time-lapse imaging using transmitted light and fluorescence microscopy. The submerged filter paper sandwich is a variant of the well-established filter paper carrier technique¹ that allows for the culturing of chick embryos fully-submerged in a liquid culture medium.