Academic Medical Center Amsterdam
2 ARTICLES PUBLISHED IN JoVE
Medicine
The Use of Pharmacological-challenge fMRI in Pre-clinical Research: Application to the 5-HT System
Anne Klomp 1, Jordi L. Tremoleda 2, Anouk Schrantee 1, Willy Gsell 2, Liesbeth Reneman 1
1Department of Radiology, Brain Imaging Center, Academic Medical Center Amsterdam, 2Biological Imaging Centre, MRC Clinical Sciences Centre, Imperial College London
The goal of this technique is to assess serotonin (5-HT) neurotransmitter function in the live and free-breathing animal with pharmacological magnetic resonance imaging (phMRI) and an intravenous challenge with a selective serotonin reuptake inhibitor (SSRI), fluoxetine.
Developmental Biology
A Submerged Filter Paper Sandwich for Long-term Ex Ovo Time-lapse Imaging of Early Chick Embryos
Manuel Schmitz *1, Ben K. A. Nelemans *1, Theodoor H. Smit 2
1Department of Orthopaedic Surgery, VU University Medical Center Amsterdam, MOVE Research Institute Amsterdam, 2Department of Anatomy, Embryology & Physiology, Academic Medical Center Amsterdam
Here, we present a new technique to culture chick embryos ex ovo for time-lapse imaging using transmitted light and fluorescence microscopy. The submerged filter paper sandwich is a variant of the well-established filter paper carrier technique1 that allows for the culturing of chick embryos fully-submerged in a liquid culture medium.
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