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University of Liège

5 ARTICLES PUBLISHED IN JoVE

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Bioengineering

The Use of a β-lactamase-based Conductimetric Biosensor Assay to Detect Biomolecular Interactions
Marylène Vandevenne *1, Mathieu Dondelinger *1, Sami Yunus 2, Astrid Freischels 1, Régine Freischels 1, Oscar Crasson 1, Noureddine Rhazi 1, Pierre Bogaerts 3, Moreno Galleni 1, Patrice Filée 4
1Life Science Department, University of Liège, 2Institute of Condensed Matter and Nanoscience, Catholic University of Louvain, 3Laboratory of Clinical Microbiology, Catholic University of Louvain, 4Biotechnology Department, CER Group

In this work, we report a new method to study protein-protein interactions using a conductimetric biosensor based on the hybrid β-lactamase technology. This method relies on release of protons upon hydrolysis of β-lactams.

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Medicine

Effects of Allogeneic Platelet-Rich Plasma (PRP) on the Healing Process of Sectioned Achilles Tendons of Rats: A Methodological Description
Laura Greimers 1, Pierre V. Drion 1, Alain Colige 2, Vincent Libertiaux 3, Vincent Denoël 3, Christelle Lecut 4, André Gothot 4, Jean-François Kaux 5
1Experimental Surgery, GIGA-R & Credec, University of Liège, 2Laboratory of Connective Tissues Biology, GIGA-R, University of Liège, 3Department Argenco, University of Liège, 4Department of Clinical Biology, University Hospital of Liège, University of Liège, 5Physical Medicine and Sport Traumatology Department, FIFA Medical Center of Excellence, University Hospital of Liège, University of Liège

This protocol describes the evaluation process of healing tendons in rats that have been injected with allogeneic platelet rich plasma (PRP) or saline solution after removing part of the Achilles tendon. The progress of tendon healing is evaluated at several time points using different types of analyses.

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Immunology and Infection

Nasal Brushing Sampling and Processing Using Digital High Speed Ciliary Videomicroscopy – Adaptation for the COVID-19 Pandemic
Noemie Bricmont 1, Lionel Benchimol 2, Anne-Lise Poirrier 2, Christine Grignet 3, Claire Seaton 4, Mark A. Chilvers 4, Marie-Christine Seghaye 5, Renaud Louis 1,6, Philippe Lefebvre 2, Celine Kempeneers 1,7
1Pneumology laboratory, I3 Group, GIGA Research Center, University of Liège, 2Department of ENT, University Hospital of Liège, 3Department for Occupational Safety and Health, Biosafety and Biosecurity Unit, University of Liège, 4Division of Respirology, Department of Pediatrics, University of British Columbia and British Columbia Children's Hospital, 5Division of Cardiology, Department of Pediatrics, University Hospital of Liège, 6Department of Pneumology, University Hospital of Liège, 7Division of Respirology, Department of Pediatrics, University Hospital of Liège

To guarantee a successful and high-quality ciliary functional analysis for PCD diagnosis, a precise and careful method for respiratory epithelium sampling and processing is essential. To continue providing PCD diagnostic service during the COVID-19 pandemic, the ciliary videomicroscopy protocol has been updated to include appropriate infection control measures.

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Behavior

SECONDs Administration Guidelines: A Fast Tool to Assess Consciousness in Brain-injured Patients
Leandro R. D. Sanz *1,2, Charlène Aubinet *1,2, Helena Cassol 1,2, Olivier Bodart 1,2, Sarah Wannez 1,2, Estelle A. C. Bonin 1,2, Alice Barra 1,2, Nicolas Lejeune 1,2,3,4, Charlotte Martial 1,2, Camille Chatelle 1,2, Didier Ledoux 5,6, Steven Laureys 1,2, Aurore Thibaut 1,2, Olivia Gosseries 1,2
1Coma Science Group, GIGA-Consciousness, GIGA research center, University of Liège, 2Centre du Cerveau, University Hospital of Liège, 3CHN William Lennox, Groupe Hospitalier Saint-Luc, 4Institute of NeuroScience, UCLouvain, 5Department of Intensive Care, University Hospital of Liège, 6Anesthesia & Intensive Care Laboratory, GIGA-Consciousness, GIGA research center, University of Liège

These guidelines can be used for the administration of the Simplified Evaluation of CONsciousness Disorders (SECONDs), a short behavioral tool developed to diagnose brain-injured patients in time-constrained settings. This scale examines command-following, communication, visual pursuit, fixation, pain localization, oriented movements and arousal.

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Environment

Tree Core Analysis with X-ray Computed Tomography
Tom De Mil 1, Jan Van den Bulcke 2,3
1Forest Is Life, TERRA Teaching and Research Centre, Gembloux Agro Bio-Tech, University of Liège, 2UGent-Woodlab, Laboratory of Wood Technology, Department of Environment, Ghent University, 3UGent Centre for X-ray Tomography (UGCT)

Here we show how to process tree cores with an X-ray computed tomography toolchain. Except for chemical extraction for some purposes, no further physical lab treatment is needed. The toolchain can be used for biomass estimations, for obtaining MXD/tree-ring width data as well as for obtaining quantitative wood anatomy data.

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