Nasal Brushing Sampling and Processing Using Digital High Speed Ciliary Videomicroscopy – Adaptation for the COVID-19 Pandemic

4.5K Views

•

09:03 min

•

November 7th, 2020

DOI :

10.3791/61949-v

November 7th, 2020

•

Noemie Bricmont¹, Lionel Benchimol², Anne-Lise Poirrier², Christine Grignet³, Claire Seaton⁴, Mark A. Chilvers⁴, Marie-Christine Seghaye⁵, Renaud Louis¹^,⁶, Philippe Lefebvre², Celine Kempeneers¹^,⁷

¹Pneumology laboratory, I3 Group, GIGA Research Center, University of Liège, ²Department of ENT, University Hospital of Liège, ³Department for Occupational Safety and Health, Biosafety and Biosecurity Unit, University of Liège, ⁴Division of Respirology, Department of Pediatrics, University of British Columbia and British Columbia Children's Hospital, ⁵Division of Cardiology, Department of Pediatrics, University Hospital of Liège, ⁶Department of Pneumology, University Hospital of Liège, ⁷Division of Respirology, Department of Pediatrics, University Hospital of Liège

Summary

Explore More Videos

Nasal Brushing

Digital High speed Videomicroscopy

Primary Ciliary Dyskinesia

Diagnostic Test

Infection Control Measures

Ciliary Functional Evaluation

Electron Microscopy

Genetic Testing

Sample Collection Procedure

Cytology Brush

Medium 199

Microbiological Safety Cabinet

Ciliated Epithelium

Chapters in this video

0:05

Introduction

1:41

Respiratory Ciliated Epithelium Sample Collection

2:35

Respiratory Ciliated Epithelium Processing and Preparation

3:55

Respiratory Ciliated Edge Selection and Recording

5:18

Ciliated Edge Analysis

6:59

Results: Representative Nasal Brush Processing Analysis

8:34

Conclusion

Related Videos

04:37

Finger-stick Blood Sampling Methodology for the Determination of Exercise-induced Lymphocyte Apoptosis

12.1K Views

07:29

Determination of Molecular Structures of HIV Envelope Glycoproteins using Cryo-Electron Tomography and Automated Sub-tomogram Averaging

41.4K Views

12:23

Granulocyte-dependent Autoantibody-induced Skin Blistering

10.4K Views

15:28

A Microscopic Phenotypic Assay for the Quantification of Intracellular Mycobacteria Adapted for High-throughput/High-content Screening

7.6K Views

10:02

Visualizing Non-lytic Exocytosis of Cryptococcus neoformans from Macrophages Using Digital Light Microscopy

11.7K Views

05:59

Nasal Wipes for Influenza A Virus Detection and Isolation from Swine

9.3K Views

09:13

Gene Expression Profiling of Infecting Microbes Using a Digital Bar-coding Platform

8.0K Views

11:36

A RAPID Method for Blood Processing to Increase the Yield of Plasma Peptide Levels in Human Blood

8.4K Views

10:03

Swab Sampling Method for the Detection of Human Norovirus on Surfaces

19.5K Views

09:15

Electrochemiluminescence Assays for Human Islet Autoantibodies

15.3K Views