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Tokyo University of agriculture and technology

10 ARTICLES PUBLISHED IN JoVE

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Neuroscience

Assaying Surface Expression of Chemosensory Receptors in Heterologous Cells
Sandeepa Dey 1, Senmiao Zhan 2, Hiroaki Matsunami 1
1Department of Molecular Genetics and Molecular Biology , Duke University, 2Department of Chemistry, Duke University

Here we demonstrate a protocol to carry out live cell staining that can be used to detect odorant receptors on the surface of HEK293T cells conveniently. In addition, it may also be used to assay for surface expression of other chemosensory receptors or GPCRs.

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Behavior

A Mouse Model of Subchronic and Mild Social Defeat Stress for Understanding Stress-induced Behavioral and Physiological Deficits
Tatsuhiko Goto 1,2, Atsushi Toyoda 1,2,3
1College of Agriculture, Ibaraki University, 2Cooperation between Agriculture and Medical Science, Ibaraki University, 3United Graduate School of Agricultural Science, Tokyo University of Agriculture and Technology

Here, methods for developing a mouse model of subchronic and mild social defeat stress are described and used to investigate the pathogenic features of depression including hyperphagia- and polydipsia-like symptoms following increased body weight.

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Behavior

Determining Ultrasonic Vocalization Preferences in Mice using a Two-choice Playback Test
Akari Asaba 1, Masahiro Kato 3, Nobuyoshi Koshida 2, Takefumi Kikusui 1
1School of Veterinary Medicine, Azabu University, 2Division of Electric and Information Engineering, Tokyo University of Agriculture and Technology, 3Kato Acoustics Consulting Office

Ultrasonic vocalizations (USVs) in mice differ depending on age, sex, condition, and genetic background. Using two ultrasound emitters broadcasting simultaneously in different locations, this two-choice test can evaluate murine recognition and preference responses to different characteristics of USVs.

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Chemistry

Construction of Models for Nondestructive Prediction of Ingredient Contents in Blueberries by Near-infrared Spectroscopy Based on HPLC Measurements
Wenming Bai 1, Norio Yoshimura 1, Masao Takayanagi 1, Jingai Che 2, Naomi Horiuchi 2, Isao Ogiwara 3
1United Graduate School of Agricultural Science, Tokyo University of Agriculture and Technology, 2Faculty of Agriculture, Tokyo University of Agriculture and Technology, 3Institute of Agriculture, Tokyo University of Agriculture and Technology

We present here a protocol to construct and validate models for nondestructive prediction of total sugar, total organic acid, and total anthocyanin content in individual blueberries by near-infrared spectroscopy.

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Neuroscience

Live-cell Measurement of Odorant Receptor Activation Using a Real-time cAMP Assay
Yuetian Zhang *1, Yi Pan *1, Hiroaki Matsunami 2,3, Hanyi Zhuang 1,4
1Department of Pathophysiology, Key Laboratory of Cell Differentiation and Apoptosis of National Ministry of Education, Shanghai Jiao Tong University School of Medicine, 2Department of Molecular Genetics and Microbiology, Duke University Medical Center, 3Department of Neurobiology, Duke Institute for Brain Sciences, Duke University Medical Center, 4Institute of Health Science, Chinese Academy of Science/Shanghai Jiao Tong University School of Medicine

Characterizing the function of odorant receptors serves an indispensable part in the deorphanization process. We describe a method to measure the activation of odorant receptors in real time using a cAMP assay.

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Environment

Tracking Infiltration Front Depth Using Time-lapse Multi-offset Gathers Collected with Array Antenna Ground Penetrating Radar
Hirotaka Saito 1, Seiichiro Kuroda 2, Toshiki Iwasaki 1, Haruyuki Fujimaki 3, Nobuhito Nagai 4, Jacopo Sala 5
1Graduate School of Agriculture, Tokyo University of Agriculture and Technology, 2National Institute for Rural Engineering, National Agriculture and Food Research Organization, 3Arid Land Research Center, Tottori University, 4GeoFive Co. Ltd, 53D-Radar

Here we present a Ground Penetrating Radar (GPR) system based on a ground-coupled, densely populated antenna array for monitoring the dynamic process of subsurface water infiltration. A time-lapse radar image of the infiltration process allowed estimating the depth of the wetting front during the course of the infiltration process.

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Neuroscience

Real-time In Vitro Monitoring of Odorant Receptor Activation by an Odorant in the Vapor Phase
Claire A. de March 1, Yosuke Fukutani 1,2, Aashutosh Vihani 1,3, Hitoshi Kida 1,4, Hiroaki Matsunami 1,3,5,6
1Department of Molecular Genetics and Microbiology, Duke University Medical Center, 2Department of Biotechnology and Life Science, Tokyo University of Agriculture and Technology, 3Department of Neurobiology, Duke University Medical Center, 4Department of Mechanical Systems, Engineering, Tokyo University of Agriculture and Technology, 5Institute of Global Innovation Research, Tokyo University of Agriculture and Technology, 6Duke Institute for Brain Sciences, Duke University

Physiologically, odorant receptors are activated by odorant molecules inhaled in the vapor phase. However, most in vitro systems utilize liquid phase odorant stimulation. Here, we present a method that allows real-time in vitro monitoring of odorant receptor activation upon odorant stimulation in vapor phase.

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Medicine

Sarcomere Shortening of Pluripotent Stem Cell-Derived Cardiomyocytes using Fluorescent-Tagged Sarcomere Proteins.
Razan E. Ahmed *1, Nawin Chanthra *1, Tatsuya Anzai 1,2, Keiichiro Koiwai 3,4, Tomoki Murakami 4, Hiroaki Suzuki 4, Yutaka Hanazono 1, Hideki Uosaki 1
1Division of Regenerative Medicine, Center for Molecular Medicine, Jichi Medical University, 2Department of Pediatrics, Jichi Medical University, 3Institute of Global Innovation Research, Tokyo University of Agriculture and Technology, 4Department of Precision Mechanics, Faculty of Science and Engineering, Chuo University

This method can be used to examine sarcomere shortening using pluripotent stem cell-derived cardiomyocytes with fluorescent-tagged sarcomere proteins.

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Environment

Mass-Rearing and Molecular Studies in Tortricidae Pest Insects
Hiroshi Arai 1, Yuna Ishitsubo 1, Madoka Nakai 1, Maki N. Inoue 1
1Department of Applied Biological Science, Tokyo University of Agriculture and Technology

The present protocol describes the rearing method of tortricid pest insects in the laboratories. The procedures to distinguish insects' sex and extract nucleic acids for high throughput sequencing are established using two tortricid pests.

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Bioengineering

Cellular Affinity of Particle-Stabilized Emulsion to Boost Antigen Internalization
Fengqiang Cao *1,2,3, Yali Ming *3,4, Weixiang Gao 3,4, Jia Ge 3, Kenji Ogino 1,2
1Graduate School of Bio-Applications and Systems Engineering, Tokyo University of Agriculture and Technology, 2Institute of Global Innovation Research, Tokyo University of Agriculture and Technology, 3State Key Laboratory of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences, 4University of Chinese Academy of Sciences

To rationally design efficient adjuvants, we developed poly-lactic-co-glycolic acid nanoparticle-stabilized Pickering emulsion (PNPE). The PNPE possessed unique softness and a hydrophobic interface for potent cellular contact and offered high-content antigen loading, improving the cellular affinity of the delivery system to antigen-presenting cells and inducing efficient internalization of antigens.

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