Sarcomere Shortening of Pluripotent Stem Cell-Derived Cardiomyocytes using Fluorescent-Tagged Sarcomere Proteins.
4.3K Views
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08:37 min
•
March 3rd, 2021
DOI :
March 3rd, 2021
•Razan E. Ahmed*1, Nawin Chanthra*1, Tatsuya Anzai1,2, Keiichiro Koiwai3,4, Tomoki Murakami4, Hiroaki Suzuki4, Yutaka Hanazono1, Hideki Uosaki1
1Division of Regenerative Medicine, Center for Molecular Medicine, Jichi Medical University, 2Department of Pediatrics, Jichi Medical University, 3Institute of Global Innovation Research, Tokyo University of Agriculture and Technology, 4Department of Precision Mechanics, Faculty of Science and Engineering, Chuo University
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Chapters in this video
0:04
Introduction
1:04
Human Pluripotent Stem Cell Differentiation
3:15
Patterned Pluripotent Stem Cell-Derived Cardiomyocyte (PSC-CM) Culture
5:07
Time-Lapse Sarcomere Imaging
5:58
Time-Lapse Imaging Analysis
6:36
Results: Representative Sarcomere Shortening Evaluation
7:56
Conclusion
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