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Jena University Hospital

12 ARTICLES PUBLISHED IN JoVE

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Neuroscience

In vivo Imaging of Optic Nerve Fiber Integrity by Contrast-Enhanced MRI in Mice
Stefanie Fischer *1, Christian Engelmann *2, Karl-Heinz Herrmann 3, Jürgen R. Reichenbach 3, Otto W. Witte 1, Falk Weih 2, Alexandra Kretz 1, Ronny Haenold 2
1Hans Berger Department of Neurology, Jena University Hospital, 2Immunology, Leibniz Institute for Age Research, Fritz Lipmann Institute, Jena, 3Institute of Diagnostic and Interventional Radiology, Medical Physics Group, Jena University Hospital

This video illustrates a method, using a clinical 3 T scanner, for contrast-enhanced MR imaging of the naïve mouse visual projection and for repetitive and longitudinal in vivo studies of optic nerve degeneration associated with acute optic nerve crush injury and chronic optic nerve degeneration in knock-out mice (p50KO).

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Medicine

Monitoring of Systemic and Hepatic Hemodynamic Parameters in Mice
Chichi Xie 1,3, Weiwei Wei 1, Tao Zhang 1, Olaf Dirsch 2, Uta Dahmen 1
1Department of General, Visceral and Vascular Surgery, Jena University Hospital, 2Institute of Pathology, Jena University Hospital, 3Reproductive Medicine Center, The First Affiliated Hospital of Wenzhou Medical University

This film demonstrates how to acquire systemic and hepatic hemodynamics in mice. The whole monitoring includes acquisition of vital parameters, systemic blood pressure, central venous pressure, common hepatic artery flow rate, and portal vein pressure as well as the portal flow rate in mice.

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Bioengineering

Fluorescence-quenching of a Liposomal-encapsulated Near-infrared Fluorophore as a Tool for In Vivo Optical Imaging
Felista L. Tansi *1, Ronny Rüger *2, Markus Rabenhold 2, Frank Steiniger 3, Alfred Fahr 2, Ingrid Hilger 1
1Experimental Radiology, Institute of Diagnostic and Interventional Radiology I, Jena University Hospital, 2Department of Pharmaceutical Technology, Friedrich-Schiller-University Jena, 3Center for Electron Microscopy, Jena University Hospital

The use of fluorophores for in vivo imaging can be greatly limited by opsonization, rapid clearance, low detection sensitivity and cytotoxic effects on the host. Encapsulation of fluorophores in liposomes by film hydration and extrusion leads to fluorescence quenching and protection which enables in vivo imaging with high detection sensitivity.

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Neuroscience

Immunohistochemistry and Multiple Labeling with Antibodies from the Same Host Species to Study Adult Hippocampal Neurogenesis
Anne Ansorg *1, Katja Bornkessel *1, Otto W. Witte 1, Anja Urbach 1
1Hans Berger Department of Neurology, Jena University Hospital

This video article illustrates a comprehensive protocol to detect and quantify all stages of adult hippocampal neurogenesis within the same tissue section. We elaborated a method to overcome the limitations of indirect multiple immunofluorescence that arise when suitable antibodies from different host species are unavailable.

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Medicine

Visualization of Vascular and Parenchymal Regeneration after 70% Partial Hepatectomy in Normal Mice
Chichi Xie 1, Weiwei Wei 1, Andrea Schenk 3, Lars Ole Schwen 3, Sara Zafarnia 2, Michael Schwier 3, Felix Gremse 2, Isabel Jank 1, Olaf Dirsch 4, Uta Dahmen 1
1Department of General, Visceral and Vascular Surgery, Jena University Hospital, 2Experimental Molecular Imaging, RWTH Aachen University, 3Fraunhofer Institute for Medical Image Computing MEVIS, 4Institute of Pathology, Klinikum Chemnitz gGmbH

Tools used for visualizing vascular regeneration require methods for contrasting the vascular trees. This film demonstrated a delicate injection technique used to achieve optimal contrasting of the vascular trees and illustrate the potential benefits resulting from a detailed analysis of the resulting specimen using µCT and histological serial sections.

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Bioengineering

A Novel Surgical Technique As a Foundation for In Vivo Partial Liver Engineering in Rat
An Wang 1, Isabel Jank 1, Weiwei Wei 1, Claudia Schindler 1, Uta Dahmen 1
1Experimental Transplantation Surgery, Department of General, Visceral and Vascular Surgery, University Hospital Jena

We establish a novel surgical technique for an in vivo single liver lobe perfusion model in rat as a prerequisite for further studying in vivo partial liver engineering in the future.

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Immunology and Infection

Measuring Phagocytosis of Aspergillus fumigatus Conidia by Human Leukocytes using Flow Cytometry
Susann Hartung 1,2, Christopher Rauh 2, Sarah Böttcher 1, Mai Thi Ngoc Hoang 1,2, Susanne Jahreis 1,2, Silke Rummler 3, Andreas Hochhaus 2, Marie von Lilienfeld-Toal 1,2
1Infections in Hematology and Oncology, Leibniz Institute for Infection Biology and Natural Product Research, 2Department for Hematology and Medical Oncology, Jena University Hospital, 3Institute for Transfusion Medicine, Jena University Hospital

This protocol provides a fast and reliable method to quantitatively measure phagocytosis of Aspergillus fumigatus conidia by human primary phagocytes using flow cytometry and to discriminate phagocytosis of conidia from mere adhesion to leukocytes.

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JoVE Core

Multiplexed Fluorescent Immunohistochemical Staining of Four Endometrial Immune Cell Types in Recurrent Miscarriage
Yiwei Zhao 1, Gene Chi Wai Man 1,2, Loucia Kit Ying Chan 1, Xi Guo 1, Yingyu Liu 3, Tao Zhang 1, Joseph Kwong 1,4, Chi Chiu Wang 1,5, Xiaoyan Chen 3, Tin Chiu Li 1
1Department of Obstetrics and Gynaecology, The Chinese University of Hong Kong, Prince of Wales Hospital, 2Department of Orthopaedics and Traumatology, The Chinese University of Hong Kong, Prince of Wales Hospital, 3Department of Obstetrics and Gynaecology, Shenzhen Baoan Women’s and Children’s Hospital, Shenzhen University, 4School of Medicine, Faculty of Medicine and Health Sciences, Keele University, 5Li Ka Shing Institute of Health Sciences; School of Biomedical Sciences, The Chinese University of Hong Kong

Despite the advancements in multiplex immunohistochemistry and multispectral imaging, characterizing the density and clustering of major immune cells simultaneously in the endometrium remains a challenge. This paper describes a detailed multiplex staining protocol and imaging for the simultaneous localization of four immune cell types in the endometrium.

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Neuroscience

Manual Segmentation of the Human Choroid Plexus Using Brain MRI
Deepthi Bannai *1,2, Yuan Cao *3,4, Matcheri Keshavan 1,2, Martin Reuter 5,6,7, Paulo Lizano 1,2,8
1Department of Psychiatry, Beth Israel Deaconess Medical Center, 2Division of Translational Neuroscience, Beth Israel Deaconess Medical Center, 3Huaxi MR Research Center (HMRRC), Department of Radiology, West China Hospital of Sichuan University, 4Department of Psychiatry and Psychotherapy, Jena University Hospital, 5AI in Medical Imaging, German Center for Neurodegenerative Diseases (DZNE), 6A. A. Martinos Center for Biomedical Imaging, Massachusetts General Hospital, 7Department of Radiology, Harvard Medical School, 8Department of Psychiatry, Harvard Medical School

Despite the crucial role of the choroid plexus in the brain, neuroimaging studies of this structure are scarce due to the lack of reliable automated segmentation tools. The present protocol aims to ensure gold-standard manual segmentation of the choroid plexus that can inform future neuroimaging studies.

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Engineering

Normothermic Ex Vivo Liver Machine Perfusion in Mouse
Haotian Chen 1, Olaf Dirsch 2, Mohamed Albadry 1,3, Paz Hernandez Ana 1, Uta Dahmen 1
1Experimental Transplantation Surgery, Department of General, Visceral and Vascular Surgery, Jena University Hospital, 2Institute of Pathology, Klinikum Chemnitz GmbH, 3Department of Pathology, Faculty of Veterinary Medicine, Menoufia University

A normothermic ex vivo liver perfusion (NEVLP) system was created for mouse livers. This system requires experience in microsurgery but allows for reproducible perfusion results. The ability to utilize mouse livers facilitates the investigation of molecular pathways to identify novel perfusate additives and enables the execution of experiments focused on organ repair.

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Immunology and Infection

Immunocompetent Alveolus-on-Chip Model for Studying Alveolar Mucosal Immune Responses
Hristina Koceva 1, Mona Amiratashani 1, Knut Rennert 2, Alexander S. Mosig 1,3,4
1Institute of Biochemistry II, Jena University Hospital, 2Dynamic42 GmbH, 3Center for Sepsis Control and Care, Jena University Hospital, 4Jena School of Microbial Communication, Friedrich Schiller University Jena

Lung-on-chip models surpass traditional 2D cultures by mimicking the air-liquid interface and endothelial cell perfusion, simulating blood flow and nutrient exchange crucial for lung physiology studies. This enhances lung research relevance, offering a dynamic, physiologically accurate environment to advance the understanding and treatment of respiratory infections.

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Immunology and Infection

Immunocompetent Intestine-on-Chip Model for Analyzing Gut Mucosal Immune Responses
Adrian Feile *1,2,3, Valentin D. Wegner *1, Martin Raasch 4, Alexander S. Mosig 1,2,3,5
1Institute of Biochemistry II, Jena University Hospital, 2Cluster of Excellence Balance of the Microverse, Friedrich Schiller University, 3Jena School of Microbial Communication, Friedrich Schiller University, 4Dynamic42 GmbH, 5Jena Center for Sepsis Control and Care, Jena University Hospital

Our detailed protocol outlines the creation and use of the advanced intestine-on-chip model, which simulates human intestinal mucosa with 3D structures and various cell types, enabling in-depth analysis of immune responses and cellular functions in response to microbial colonization.

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