This protocol demonstrates a straightforward and robust method to study in situ axon growth and growth cone dynamics. It describes how to prepare ex vivo physiologically relevant acute brain slices and provides a user-friendly analysis pipeline.

in-situ-visualization-axon-growth-growth-cone-dynamics-acute-ex-vivo

In Situ Visualization of Axon Growth and Growth Cone Dynamics in Acute Ex Vivo Embryonic Brain Slice Cultures

During neuronal development, axons navigate the cortical environment to reach their final destinations and establish synaptic connections. Growth cones ...

Deutsches Zentrum für Neurodegenerative Erkrankungen (DZNE)

1 ARTICLES PUBLISHED IN JoVE

Neuroscience