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University of Warwick

13 ARTICLES PUBLISHED IN JoVE

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Biology

Identification of Post-translational Modifications of Plant Protein Complexes
Sophie J. M. Piquerez 1, Alexi L. Balmuth 2, Jan Sklenář 2, Alexandra M.E. Jones 1,2, John P. Rathjen 3, Vardis Ntoukakis 1
1School of Life Sciences, University of Warwick, 2The Sainsbury Laboratory, Norwich Research Park, 3Research School of Biology, The Australian National University

We describe here a protocol for the purification and characterization of plant protein complexes. We demonstrate that by immunoprecipitating a single protein within a complex, so we can identify its post-translational modifications and its interacting partners.

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Engineering

Resonance Raman Spectroscopy of Extreme Nanowires and Other 1D Systems
David C. Smith 1, Joseph H. Spencer 1, Jeremy Sloan 2, Liam P. McDonnell 1, Harrison Trewhitt 2, Reza J. Kashtiban 2, Eric Faulques 3
1School of Physics and Astronomy, University of Southampton, 2Department of Physics, University of Warwick, 3Institut des Matériaux Jean Rouxel, University of Nantes CNRS

The paper describes a method for producing extreme nanowires by melt infiltration into carbon nanotubes and how 1D systems may be characterized and investigated using Resonance Raman Spectroscopy to determine vibrational and optical excitation energies.

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Chemistry

Assessment of Boron Doped Diamond Electrode Quality and Application to In Situ Modification of Local pH by Water Electrolysis
Tania L. Read 1, Julie V. Macpherson 1
1Warwick Electrochemistry and Interfaces Group, Department of Chemistry, University of Warwick

A protocol is described for the characterization of the key electrochemical parameters of a boron doped diamond (BDD) electrode and subsequent application for in situ pH generation experiments.

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Bioengineering

Using Tomoauto: A Protocol for High-throughput Automated Cryo-electron Tomography
Dustin R. Morado 1, Bo Hu 1, Jun Liu 1
1Department of Pathology and Laboratory Medicine, University of Texas Health Science Center at Houston

We present a protocol on how to utilize high-throughput cryo-electron tomography to determine high resolution in situ structures of molecular machines. The protocol permits large amounts of data to be processed, avoids common bottlenecks and reduces resource downtime, allowing the user to focus on important biological questions.

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JoVE Core

Knowledge Based Cloud FE Simulation of Sheet Metal Forming Processes
Du Zhou 1, Xi Yuan 1, Haoxiang Gao 1, Ailing Wang 1, Jun Liu 1, Omer El Fakir 1, Denis J. Politis 1, Liliang Wang 1, Jianguo Lin 1
1Department of Mechanical Engineering, Imperial College London

The following paper presents a novel FE simulation technique (KBC-FE), which reduces computational cost by performing simulations on a cloud computing environment, through the application of individual modules. Moreover, it establishes a seamless collaborative network between world leading scientists, enabling the integration of cutting edge knowledge modules into FE simulations.

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Engineering

Digital Printing of Titanium Dioxide for Dye Sensitized Solar Cells
Ruth Cherrington 1, Benjamin Michael Wood 1, Iulia Salaoru 1, Vannessa Goodship 1
1Warwick Manufacturing Group, University of Warwick

This paper investigates the suitability of inkjet printing for the manufacturing of dye-sensitized solar cells. A binder-free TiO2 nanoparticle ink was formulated and printed onto a FTO glass substrate. The printed layer was fabricated into a cell with an active area of 0.25 cm2 and an efficiency of 3.5%.

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Engineering

Inkjet-printed Polyvinyl Alcohol Multilayers
Iulia Salaoru 1,2, Zuoxin Zhou 2, Peter Morris 3, Gregory J. Gibbons 2
1Emerging Technologies Research Centre (EMTERC), De Montfort University, 2WMG, University of Warwick, 3PVOH Polymer Ltd.

An inkjet printer was used to manufacture polyvinyl alcohol multilayers. Polyvinyl alcohol water-based ink was formulated, and the main physical properties were investigated.

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Developmental Biology

In Vitro Growth of Mouse Preantral Follicles Under Simulated Microgravity
Shen Zhang 1, Yonggen Wu 1, Yimin Weng 2, Zhihui Xu 1, Wenmin Chen 3, Dahan Zheng 4, Wei Lin 5, Jun Liu 6, Ying Zhou 1,7
1Reproductive Medicine Center, The First Affiliated Hospital of Wenzhou Medical University, 2Department of Orthopaedics, The Second Affiliated Hospital of Wenzhou Medical University, 3Department of Obstetrics, The First Affiliated Hospital of Wenzhou Medical University, 4School of Laboratory Medicine and Life Science, Wenzhou Medical University, 5School of Pharmaceutical Science, Wenzhou Medical University, 6Stem Cells and Genetic Engineering Group, AgriBioscience Research Centre, Department of Economic Development, Jobs, Transport and Resources, 7Department of Histology and Embryology, Wenzhou Medical University

A highly promising technique to generate tissue constructs without using matrix is to culture cells in a simulated microgravity condition. Using a rotary culture system, we examined ovarian follicle growth and oocyte maturation in terms of follicle survival, morphology, growth, and oocyte function under the simulated microgravity condition.

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Bioengineering

Optimized Setup and Protocol for Magnetic Domain Imaging with In Situ Hysteresis Measurement
Jun Liu 1, John Wilson 2, Claire Davis 1, Anthony Peyton 2
1Advanced Steel Research Centre, Warwick Manufacturing Group, University of Warwick, 2School of Electrical and Electronic Engineering, University of Manchester

This paper elaborates the sample and sensor preparation procedures and the protocols for using the test rig particularly for dynamic domain imaging with in situ BH measurements in order to achieve optimal domain pattern quality and accurate BH measurements.

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Immunology and Infection

Applying Live Cell Imaging and Cryo-Electron Tomography to Resolve Spatiotemporal Features of the Legionella pneumophila Dot/Icm Secretion System
David Chetrit 1, Donghyun Park 1,2, Bo Hu 3, Jun Liu 1,2, Craig R. Roy 1
1Department of Microbial Pathogenesis, Boyer Center for Molecular Medicine, Yale University School of Medicine, 2Microbial Sciences Institute, Yale University, 3Department of Microbiology and Molecular Genetics, McGovern Medical School, The University of Texas Health Science Center at Houston

Imaging of bacterial cells is an emerging systems biology approach focused on defining static and dynamic processes that dictate the function of large macromolecular machines. Here, integration of quantitative live cell imaging and cryo-electron tomography is used to study Legionella pneumophila type IV secretion system architecture and functions.

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Immunology and Infection

Antibiotic Efficacy Testing in an Ex vivo Model of Pseudomonas aeruginosa and Staphylococcus aureus Biofilms in the Cystic Fibrosis Lung
Niamh E. Harrington *1, Esther Sweeney *1, Ilyas Alav 1,2, Freya Allen 1, John Moat 1,3, Freya Harrison 1
1School of Life Science, University of Warwick, 2Institute of Microbiology and Infection, University of Birmingham, 3Warwick Antimicrobial Screening Facility, School of Life Science, University of Warwick

This workflow can be used to perform antibiotic susceptibility testing using an established ex vivo model of bacterial biofilm in the lungs of individuals with cystic fibrosis. Use of this model could enhance the clinical validity of MBEC (minimal biofilm eradication concentration) assays.

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JoVE Core

Microfluidic Device for the Separation of Non-Metastatic (MCF-7) and Non-Tumor (MCF-10A) Breast Cancer Cells Using AC Dielectrophoresis
Ateeq ur Rehman 1, Rahman S. Zabibah 2, Samira Kharratian 3, Adil Mustafa 4,5
1Department of Biomedical Engineering, Foundation University Islamabad, 2College of Medical Technology, The Islamic University Najaf, 3Faculty of Mechanical Engineering, University of Tabriz, 4Department of Engineering Mathematics, University of Bristol, 5School of Life Sciences, University of Warwick

Breast cancer cells exhibit different dielectric properties compared to non-tumor breast epithelial cells. It has been hypothesized that, based on this difference in dielectric properties, the two populations can be separated for immunotherapy purposes. To support this, we model a microfluidic device to sort MCF-7 and MCF-10A cells.

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Biology

Reconstitution of Membrane-Tethered Minimal Actin Cortices on Supported Lipid Bilayers
Darius Vasco Köster 1, Abrar Bhat 2, Sankarshan Talluri 2, Satyajit Mayor 2
1Centre for Mechanochemical Cell Biology and Division of Biomedical Sciences, Warwick Medical School, University of Warwick, 2National Centre for Biological Sciences, Tata Institute of Fundamental Research

This protocol describes the formation of supported lipid bilayers and the addition of cytoskeletal filaments and motor proteins to study the dynamics of reconstituted, membrane-tethered cytoskeletal networks using fluorescence microscopy.

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