Applying Live Cell Imaging and Cryo-Electron Tomography to Resolve Spatiotemporal Features of the Legionella pneumophila Dot/Icm Secretion System
6.9K Views
•
09:12 min
•
March 10th, 2020
DOI :
March 10th, 2020
•Chapters in this video
0:04
Introduction
0:55
Live Cell Imaging of L. pneumophila with Fluorescently Tagged Dot/Icm Components
2:47
Quantification of Polar Localization and Dynamics of Dot/Icm Components
5:36
Detection of sfGFP Mass Density with Cryo-ET
6:23
Results: Imaging Dot/Icm Secretion Systems in Intact Bacteria
8:17
Conclusion
Related Videos
13:43
Correlative Microscopy for 3D Structural Analysis of Dynamic Interactions
14.0K Views
09:24
Visualizing Surface T-Cell Receptor Dynamics Four-Dimensionally Using Lattice Light-Sheet Microscopy
7.9K Views
09:09
A Label-free Technique for the Spatio-temporal Imaging of Single Cell Secretions
8.5K Views
11:33
Using Tomoauto: A Protocol for High-throughput Automated Cryo-electron Tomography
10.9K Views
14:23
Electron Cryotomography of Bacterial Cells
25.4K Views
06:33
Three-dimensional Imaging of Bacterial Cells for Accurate Cellular Representations and Precise Protein Localization
9.9K Views
08:01
High-Resolution Three-Dimensional Whole-Organ Tomography of Microbial Infections
843 Views
09:47
Visualization of DNA Compaction in Cyanobacteria by High-voltage Cryo-electron Tomography
9.1K Views
08:20
Sample Preparation by 3D-Correlative Focused Ion Beam Milling for High-Resolution Cryo-Electron Tomography
3.2K Views
08:37
Visualization of Organelles In Situ by Cryo-STEM Tomography
2.0K Views
Copyright © 2025 MyJoVE Corporation. All rights reserved