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JoVE Journal

Immunology and Infection

This content is Open Access.

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Antibiotic Efficacy Testing in an Ex vivo Model of Pseudomonas aeruginosa and Staphylococcus aureus Biofilms in the Cystic Fibrosis Lung

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09:26 min

January 22nd, 2021

January 22nd, 2021

6,433 Views

0:04

Introduction

0:52

Dissection and Infection of Ex Vivo Pig Lung (EVPL) Tissue

4:45

Determination of Antibiotic Efficacy

6:39

Results: Screening Bacterial Isolates for Antibiotic Susceptibility with the EVPL Model

8:49

Conclusion

Transcript

This workflow can be used to perform antibiotic susceptibility testing using an established ex vivo model of bacterial biofilm in the lungs of individuals with cystic fibrosis. Use of this model could enhance the clinical validity of MBEC (minimal biofilm eradication concentration) assays.

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