institut imagine

2 ARTICLES PUBLISHED IN JoVE

Immunology and Infection

Development and Validation of an Ultrasensitive Single Molecule Array Digital Enzyme-linked Immunosorbent Assay for Human Interferon-α

Alba Llibre^*1,2, Vincent Bondet^*1,2, Mathieu P. Rodero³, David Hunt⁴, Yanick J. Crow^3,5, Darragh Duffy^1,2

¹Immunobiology of Dendritic Cells, Institut Pasteur, ²INSERM U1223, ³Laboratory of Neurogenetics and Neuroinflammation, INSERM UMR1163, Institut Imagine, ⁴MRC Human Genetics Unit, MRC Institute of Genetics and Molecular Medicine, University of Edinburgh, ⁵Manchester Centre for Genomic Medicine, University of Manchester

Here we present a protocol to describe the development and validation of a single molecule array digital ELISA assay, which enables the ultra-sensitive detection of all IFN-α subtypes in human samples.

Neuroscience

Behavioral And Physiological Analysis In A Zebrafish Model Of Epilepsy

Hortense de Calbiac^*1,2, Adriana Dabacan^*3, Raul Muresan³, Edor Kabashi^1,2, Sorana Ciura^1,2

¹University Paris Descartes Hospital Necker-Enfants Malades, Institut Imagine, ²Institut du Cerveau et de la Moelle épinière - ICM, Sorbonne Universités Paris, ³Transylvanian Institute of Neuroscience (TINS)

Here, we present a protocol for the development and the characterization of a zebrafish model of epilepsy resulting from the transient inhibition of the DEPDC5 gene.