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Jiangnan University

13 ARTICLES PUBLISHED IN JoVE

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Biology

Micro-Mechanical Characterization of Lung Tissue Using Atomic Force Microscopy
Fei Liu 1, Daniel J. Tschumperlin 1
1Molecular and Integrative Physiological Sciences, Department of Environmental Health, Harvard School of Public Health

The stiffness of the extracellular matrix strongly influences multiple behaviors of adherent cells. Matrix stiffness varies spatially throughout a tissue, and undergoes modification in various disease conditions. Here we develop methods to characterize spatial variations in stiffness in normal and fibrotic mouse lung tissue using atomic force microscopy microindentation.

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Cancer Research

Fabrication of Tongue Extracellular Matrix and Reconstitution of Tongue Squamous Cell Carcinoma In Vitro
Yupeng Yao 1, Weifan Lin 1, Yan Zhang 1
1Key Laboratory of Gene Engineering of the Ministry of Education, State Key Laboratory of Biocontrol, School of Life Sciences, Sun Yat-sen University

A method is shown here for the preparation of the tongue extracellular matrix (TEM) with efficient decellularization. The TEM can be used as functional scaffolds for the reconstruction of a tongue squamous cell carcinoma (TSCC) model under static or stirred culture conditions.

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Engineering

O-cresol Concentration Online Measurement Based On Near Infrared Spectroscopy Via Partial Least Square Regression
Zihao Chen 1, Niannian Zheng 1, Xiaoli Luan 1, Fei Liu 1
1Key Laboratory for Advanced Process Control of Light Industry of the Ministry of Education, Institute of Automation, Jiangnan University

The protocol describes a method of predicting o-cresol concentration during the production of polyphenylene ether using near-infrared spectroscopy and partial least squares regression. To describe the process more clearly and completely, an example of predicting the o-cresol concentration during the production of polyphenylene is used to clarify the steps.

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Cancer Research

Three-Dimensional Bone Extracellular Matrix Model for Osteosarcoma
Yu Zhang 1, Yupeng Yao 1, Yan Zhang 1
1MOE Key Laboratory of Gene Function and Regulation, School of Life Sciences, Sun Yat-sen University

The bone extracellular matrix (BEM) model for osteosarcoma (OS) is well established and shown here. It can be used as a suitable scaffold for mimicking primary tumor growth in vitro and providing an ideal model for studying the histologic and cytogenic heterogeneity of OS.

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Biochemistry

Preparation of High-Quality Fermented Fish Product
Fang Yang *1, Lu-Lu Zhu *1, Yu-Duan Diao 1, Pei Gao 1, Da-Wei Yu 1, Pei-Pei Yu 1, Qi-Xing Jiang 1, Yan-Shun Xu 1, Wen-Shui Xia 1, Xiao-Bei Zhan 2
1State Key Laboratory of Food Science and Technology, School of Food Science and Technology, Collaborative Innovation Center of Food Safety and Quality Control in Jiangsu Province, Jiangnan University, 2The Key Laboratory of Carbohydrate Chemistry and Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University

The goal of the protocol is to provide an industrialized fish fermentation technique based on inoculation of Saccharomyces cerevisiae.

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Chemistry

Cercosporin-Photocatalyzed [4+1]- and [4+2]-Annulations of Azoalkenes Under Mild Conditions
Philippe Icyishaka *1, Chenxing Li *1, Liushen Lu 1, Wenhao Bao 1, Jia Li 1, Yan Zhang 2, Yijian Rao 1
1Key Laboratory of Carbohydrate Chemistry and Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, 2School of Pharmaceutical Science, Jiangnan University

New routes for the synthesis of nitrogen-containing heterocycles utilizing cercosporin as a metal-free photocatalyst were developed.

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Biology

Three-Dimensional, Serum-Free Culture System for Lacrimal Gland Stem Cells
Hongzi Chen 1, Pan Huang 1, Yan Zhang 1
1MOE Key Laboratory of Gene Function and Regulation, School of Life Sciences, Sun Yat-sen University

The three-dimensional, serum-free culture method for adult lacrimal gland (LG) stem cells is well established for the induction of LG organoid formation and differentiation into acinar or ductal-like cells.

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Developmental Biology

Fixation of Embryonic Mouse Tissue for Cytoneme Analysis
Eric T. Hall 1, Christina A. Daly 1,2, Yan Zhang 1, Miriam E. Dillard 1, Stacey K. Ogden 1
1Department of Cell and Molecular Biology, St. Jude Children’s Research Hospital, 2St. Jude Graduate School of Biomedical Sciences

Here, an optimized step-by-step protocol is provided for fixation, immunostaining, and sectioning of embryos to detect specialized signaling filopodia called cytonemes in developing mouse tissues.

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Biology

Protocols for CRISPR/Cas9 Mutagenesis of the Oriental Fruit Fly Bactrocera dorsalis
Jinxi Yuan 1, Jie Zhang 1, Yan Zhang 1, WuYun QiQiGe 1, Wei Liu 2, Shanchun Yan 1, Guirong Wang 2
1Key Laboratory of Sustainable Forest Ecosystem Management - Ministry of Education, Northeast Forestry University, 2Shenzhen Branch, Guangdong Laboratory of Lingnan Modern Agriculture, Genome Analysis Laboratory of the Ministry of Agriculture and Rural Affairs, Agricultural Genomics Institute at Shenzhen, Chinese Academy of Agricultural Sciences

This paper presents the step-by-step protocols for CRISPR/Cas9 mutagenesis of the Oriental fruit fly Bactrocera dorsalis. Detailed steps provided by this standardized protocol will serve as a useful guide for generating mutant flies for functional gene studies in B. dorsalis.

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Cancer Research

Measurement of the Compressibility of Cell and Nucleus Based on Acoustofluidic Microdevice
Qibin Fu 1, Yan Zhang 1, Tuchen Huang 1, Yang Liu 1
1Sino-French Institute of Nuclear Engineering and Technology, Sun Yat-sen University

Here a protocol is presented to build a fast and non-destructive system for measuring cell or nucleus compressibility based on acoustofluidic microdevice. Changes in mechanical properties of tumor cells after epithelial-mesenchymal transition or ionizing radiation were investigated, demonstrating the application prospect of this method in scientific research and clinical practice.

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Medicine

Establishing a Silicosis Rat Model via Exposure of Whole-Body to Respirable Silica
Fuyu Jin 1, Yaqian Li 1, Tian Li 1, Xinyu Yang 1, Wenchen Cai 1, Shifeng Li 1, Xuemin Gao 1, Fang Yang 1, Hong Xu 1, Heliang Liu 1
1Hebei Key Laboratory for Organ Fibrosis Research, School of Public Health, North China University of Science and Technology

This study describes a technique to establish a silicosis rat model with the inhalation of silica through the whole body in an inhalation chamber. The rats with silicosis could closely mimic the pathological process of human silicosis in an easy, cost-effective manner with good repeatability.

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Immunology and Infection

Adoptive Transfer of IL-33-Stimulated Macrophages into Bleomycin-Induced Mouse Models to Study Their Effect on Idiopathic Pulmonary Fibrosis In Vivo
Xiaorun Zhai *1, Jiao Li *1, Yunjuan Nie 1
1Department of Basic Medicine, Wuxi School of Medicine, Jiangnan University

This protocol describes the isolation of pulmonary interstitial macrophages (IMs) and their adoptive transfer after IL-33 stimulation of the lung alveoli in a mouse model, which can facilitate the in vivo study of idiopathic pulmonary fibrosis (IPF).

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Recent Advances in Methods for Analyzing Lipids and Fatty Acids
Liang Zhang 1, Zhongpeng Guo 1
1National Engineering Research Center of Cereal Fermentation and Food Biomanufacturing, Jiangnan University

Recent Advances in Methods for Analyzing Lipids and Fatty Acids

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