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Fixation of Embryonic Mouse Tissue for Cytoneme Analysis

DOI :

10.3791/64100-v

8:46 min

June 16th, 2022

June 16th, 2022

1,828 Views

1Department of Cell and Molecular Biology, St. Jude Children’s Research Hospital, 2St. Jude Graduate School of Biomedical Sciences

Here, an optimized step-by-step protocol is provided for fixation, immunostaining, and sectioning of embryos to detect specialized signaling filopodia called cytonemes in developing mouse tissues.

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Fixation

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