Korea university of science and technology

3 ARTICLES PUBLISHED IN JoVE

Neuroscience

Intracerebroventricular Injection of Amyloid-β Peptides in Normal Mice to Acutely Induce Alzheimer-like Cognitive Deficits

Hye Yun Kim^1,2, Dongkeun K. Lee¹, Bo-Ryehn Chung^1,3, Hyunjin V. Kim^1,4, YoungSoo Kim^1,4

¹Center for Neuro-Medicine, Korea Institute of Science and Technology, ²Research Institute, GoshenBiotech, Inc., ³Department of Chemical and Biological Engineering, Princeton University, ⁴Biological Chemistry Program, Korea University of Science and Technology

The amyloid-β (Aβ)-injected animal model enables the administration of a defined quantity and species of Aβ fragments and reduces individual differences within each study group. This protocol describes the intracerebroventricular (ICV) injection of Aβ without stereotactic instruments, enabling the production of Alzheimer-like behavioral abnormalities in normal mice.

Biochemistry

Assay for Phosphorylation and Microtubule Binding Along with Localization of Tau Protein in Colorectal Cancer Cells

Md Nazmul Huda^1,2, Erdenedolgor Erdene-Ochir^1,2, Cheol-Ho Pan^1,2

¹Systems Biotechnology Research Center, KIST Gangneung Institute of Natural Products, ²Division of Bio-Medical Science & Technology, KIST School, Korea University of Science and Technology

This manuscript describes standard protocols for measuring tau hyperphosphorylation, measuring tau binding to microtubules, and localization of intracellular tau following drug treatments. These protocols can be used repetitively for screening drugs or other compounds that target tau hyperphosphorylation or microtubule binding.

Biology

Quantification, Viability Assessment, and Visualization Strategies for Acinetobacter Biofilms

Joo-Sung Kim^1,2, Jihoon Lim³

¹Korea Food Research Institute, ²Department of Food Biotechnology, Korea University of Science and Technology, ³Advanced Radiation Technology Institute, Korea Atomic Energy Research Institute

This protocol describes the preparation of the inoculum, the biofilm quantification on microtiter plates using crystal violet dye, the viable count in biofilms, and the visualization of biofilms of Acinetobacter.