Hunan University

5 ARTICLES PUBLISHED IN JoVE

Genetics

Single Molecule Analysis of Laser Localized Psoralen Adducts

Jing Huang¹, Himabindu Gali¹, Julia Gichimu¹, Marina A. Bellani¹, Durga Pokharel¹, Manikandan Paramasivam¹, Michael M. Seidman¹

¹Laboratory of Molecular Gerontology, National Institute on Aging, National Institutes of Health

Lasers are frequently used in studies of the cellular response to DNA damage. However, they generate lesions whose spacing, frequency, and collisions with replication forks are rarely characterized. Here, we describe an approach that enables the determination of these parameters with laser localized interstrand crosslinks.

Education

The Modular Design and Production of an Intelligent Robot Based on a Closed-Loop Control Strategy

Libo Zhang¹, Junjie Zhu², Hao Ren², Dongdong Liu³, Dan Meng⁴, Yanjun Wu¹, Tiejian Luo⁵

¹Institute of Software, Chinese Academy of Sciences, ²Hunan University, ³Fuyang Normal University, ⁴Anhui Normal University, ⁵University of Chinese Academy of Sciences

We present a protocol on modular design and production of intelligent robots to help scientific and technical workers design intelligent robots with special production tasks based on personal needs and individualized design.

Cancer Research

A Time-lapse, Label-free, Quantitative Phase Imaging Study of Dormant and Active Human Cancer Cells

Jing Huang^*1,2, Peng Guo^*1,2, Marsha A. Moses^1,2

¹Vascular Biology Program, Boston Children's Hospital, ²Department of Surgery, Harvard Medical School and Boston Children's Hospital

Dormant and active cancer cell phenotypes were characterized using quantitative phase imaging. Cell proliferation, migration, and morphology assays were integrated and analyzed in one simple method.

Biology

Visualization of Replisome Encounters with an Antigen Tagged Blocking Lesion

Jing Zhang^*1, Jing Huang^*2, Ishani Majumdar¹, Ryan C. James³, Julia Gichimu¹, Manikandan Paramasivam⁴, Durga Pokharel⁵, Himabindu Gali⁶, Marina A. Bellani¹, Michael M Seidman¹

¹Laboratory of Molecular Gerontology, National Institute on Aging, National Institutes of Health, ²Institute of Chemical Biology and Nanomedicine, State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Biology, Hunan University, ³Department of Molecular Biology and Genetics, Cornell University, ⁴Department of Cellular and Molecular Medicine, University of Copenhagen, ⁵Horizon Discovery, ⁶Boston University School of Medicine

While replication fork collisions with DNA adducts can induce double strand breaks, less is known about the interaction between replisomes and blocking lesions. We have employed the proximity ligation assay to visualize these encounters and to characterize the consequences for replisome composition.

Medicine

Organ Ischemia-Reperfusion Injury by Simulating Hemodynamic Changes in Rat Liver Transplant Model

Yuan Yuan¹, Meng-hua Chen¹, Jing Huang², Yuan Tian², Ke Qin³, Zhang Yuan¹, Wen-yan Wang¹, Zhi-jiang Wu¹, Xin-yue Tian¹, Yubin Zhang⁴

¹Department of Intensive Care Unit, The Second Affiliated Hospital of Guangxi Medical University, ²Department of hepatobiliary surgery, The Affiliated Ningbo Medical Center Lihuili Hospital of Medical School of Ningbo University, ³Department of transplantation, The Second Affiliated Hospital of Guangxi Medical University, ⁴Digital Technology and Engineering college, Ningbo University of Finance & Economics

This paper provides a detailed description of how to build an animal model of the anhepatic phase (liver ischemia) in rats to facilitate basic research into ischemia-reperfusion injury after liver transplantation.