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Single Molecule Analysis of Laser Localized Psoralen Adducts

DOI :

10.3791/55541-v

April 20th, 2017

April 20th, 2017

6,492 Views

1Laboratory of Molecular Gerontology, National Institute on Aging, National Institutes of Health

Lasers are frequently used in studies of the cellular response to DNA damage. However, they generate lesions whose spacing, frequency, and collisions with replication forks are rarely characterized. Here, we describe an approach that enables the determination of these parameters with laser localized interstrand crosslinks.

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Keywords Single Molecule Analysis

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