Northeast Forestry University

11 ARTICLES PUBLISHED IN JoVE

Biology

Intracavernosal Pressure Recording to Evaluate Erectile Function in Rodents

Feng Pan^*2, Jie Zhang^*3, Yuyan Liu¹, Liangsheng Lu¹, Xuefeng Qiu⁴, Kangtai Lv⁵, Qipeng Zhang¹

¹State Key Laboratory of Pharmaceutical Biotechnology, School of Life Sciences, Jiangsu Engineering Research Center for microRNA Biology and Biotechnology, Nanjing University, ²Department of Andrology, The Affiliated Obstetrics and Gynecology Hospital of Nanjing Medical University, Nanjing Maternity and Child Health Care Hospital, ³School of Pharmaceutical Sciences, Nanjing Tech University, ⁴Department of Urology, Affiliated Drum Tower Hospital, School of Medicine, Nanjing University, ⁵Department of Ultrasound, Nanjing Qixia District Maternity and Child Health Care Hospital

Intracavernosal pressure recording (ICP) is an important method to evaluate the erectile function of experimental animals. Here, a detailed protocol is demonstrated for the recording procedure of ICP by catheterizing the crura penis and then electrically stimulating the cavernous nerves in rats.

Cancer Research

Fabrication of Tongue Extracellular Matrix and Reconstitution of Tongue Squamous Cell Carcinoma In Vitro

Yupeng Yao¹, Weifan Lin¹, Yan Zhang¹

¹Key Laboratory of Gene Engineering of the Ministry of Education, State Key Laboratory of Biocontrol, School of Life Sciences, Sun Yat-sen University

A method is shown here for the preparation of the tongue extracellular matrix (TEM) with efficient decellularization. The TEM can be used as functional scaffolds for the reconstruction of a tongue squamous cell carcinoma (TSCC) model under static or stirred culture conditions.

Biology

Stomata Tape-Peel: An Improved Method for Guard Cell Sample Preparation

Sheldon Lawrence II^1,2,3, Quiying Pang^2,3,4, Wenwen Kong^2,3, Sixue Chen^1,2,3,5

¹Plant Molecular and Cellular Biology Program, University of Florida, ²Department of Biology, University of Florida, ³Genetics Institute (UFGI), University of Florida, ⁴Alkali Soil Natural Environmental Science Center, Northeast Forestry University, ⁵Interdisciplinary Center for Biotechnology Research (ICBR), University of Florida

This protocol describes a method of preparing enriched stomatal guard cells that is useful for physiological and other biological studies.

Cancer Research

Three-Dimensional Bone Extracellular Matrix Model for Osteosarcoma

Yu Zhang¹, Yupeng Yao¹, Yan Zhang¹

¹MOE Key Laboratory of Gene Function and Regulation, School of Life Sciences, Sun Yat-sen University

The bone extracellular matrix (BEM) model for osteosarcoma (OS) is well established and shown here. It can be used as a suitable scaffold for mimicking primary tumor growth in vitro and providing an ideal model for studying the histologic and cytogenic heterogeneity of OS.

Medicine

Immunoglobulin G N-Glycan Analysis by Ultra-Performance Liquid Chromatography

Di Liu^*1, Xizhu Xu^*2, Yuejin Li², Jie Zhang¹, Xiaoyu Zhang¹, Qihuan Li¹, Haifeng Hou², Dong Li², Wei Wang^1,2,3, Youxin Wang¹

¹Beijing Key Laboratory of Clinical Epidemiology, School of Public Health, Capital Medical University, ²School of Public Health, Shandong First Medical University & Shandong Academy of Medical Sciences, ³School of Medical and Health Sciences, Edith Cowan University

Immunoglobulin G (IgG) N-glycan is characterized using hydrophilic interaction chromatography UPLC. In addition, the structure of IgG N-glycan is clearly separated. Presented here is an introduction to this experimental method so that it can be widely used in research settings.

JoVE Core

A Reversible Silicon Oil-Induced Ocular Hypertension Model in Mice

Jie Zhang^1,2, Fang Fang^1,3, Liang Li¹, Haoliang Huang¹, Hannah C. Webber¹, Yang Sun^1,4, Vinit B. Mahajan^1,4, Yang Hu¹

¹Department of Ophthalmology, Stanford University School of Medicine, ²Department of Ophthalmology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, ³Department of Ophthalmology, Second Xiangya Hospital of Central South University, ⁴Department of Ophthalmology, Veterans Affairs Palo Alto Health Care

Here, we present a protocol to induce ocular hypertension and glaucomatous neurodegeneration in mouse eyes by intracameral injection of silicone oil and the procedure for silicone oil removal from the anterior chamber to return elevated intraocular pressure to normal.

Chemistry

Cercosporin-Photocatalyzed [4+1]- and [4+2]-Annulations of Azoalkenes Under Mild Conditions

Philippe Icyishaka^*1, Chenxing Li^*1, Liushen Lu¹, Wenhao Bao¹, Jia Li¹, Yan Zhang², Yijian Rao¹

¹Key Laboratory of Carbohydrate Chemistry and Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, ²School of Pharmaceutical Science, Jiangnan University

New routes for the synthesis of nitrogen-containing heterocycles utilizing cercosporin as a metal-free photocatalyst were developed.

Biology

Three-Dimensional, Serum-Free Culture System for Lacrimal Gland Stem Cells

Hongzi Chen¹, Pan Huang¹, Yan Zhang¹

¹MOE Key Laboratory of Gene Function and Regulation, School of Life Sciences, Sun Yat-sen University

The three-dimensional, serum-free culture method for adult lacrimal gland (LG) stem cells is well established for the induction of LG organoid formation and differentiation into acinar or ductal-like cells.

Developmental Biology

Fixation of Embryonic Mouse Tissue for Cytoneme Analysis

Eric T. Hall¹, Christina A. Daly^1,2, Yan Zhang¹, Miriam E. Dillard¹, Stacey K. Ogden¹

¹Department of Cell and Molecular Biology, St. Jude Children’s Research Hospital, ²St. Jude Graduate School of Biomedical Sciences

Here, an optimized step-by-step protocol is provided for fixation, immunostaining, and sectioning of embryos to detect specialized signaling filopodia called cytonemes in developing mouse tissues.

Biology

Protocols for CRISPR/Cas9 Mutagenesis of the Oriental Fruit Fly Bactrocera dorsalis

Jinxi Yuan¹, Jie Zhang¹, Yan Zhang¹, WuYun QiQiGe¹, Wei Liu², Shanchun Yan¹, Guirong Wang²

¹Key Laboratory of Sustainable Forest Ecosystem Management - Ministry of Education, Northeast Forestry University, ²Shenzhen Branch, Guangdong Laboratory of Lingnan Modern Agriculture, Genome Analysis Laboratory of the Ministry of Agriculture and Rural Affairs, Agricultural Genomics Institute at Shenzhen, Chinese Academy of Agricultural Sciences

This paper presents the step-by-step protocols for CRISPR/Cas9 mutagenesis of the Oriental fruit fly Bactrocera dorsalis. Detailed steps provided by this standardized protocol will serve as a useful guide for generating mutant flies for functional gene studies in B. dorsalis.

Cancer Research

Measurement of the Compressibility of Cell and Nucleus Based on Acoustofluidic Microdevice

Qibin Fu¹, Yan Zhang¹, Tuchen Huang¹, Yang Liu¹

¹Sino-French Institute of Nuclear Engineering and Technology, Sun Yat-sen University

Here a protocol is presented to build a fast and non-destructive system for measuring cell or nucleus compressibility based on acoustofluidic microdevice. Changes in mechanical properties of tumor cells after epithelial-mesenchymal transition or ionizing radiation were investigated, demonstrating the application prospect of this method in scientific research and clinical practice.