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Yangzhou University

11 ARTICLES PUBLISHED IN JoVE

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Biochemistry

Biosynthesis of a Flavonol from a Flavanone by Establishing a One-pot Bienzymatic Cascade
Zhiping Zhang 1, Shuhang Fan 1, Zhiqiang Chen 1, Yanzhi He 1, Mengfei Huang 1, Li Ding 1, Yanyan Zhang 5, Lin Chen 1, Xinyue Zhang 1,2,3,4
1College of Bioscience and Biotechnology, Yangzhou University, 2Key Laboratory of Prevention and Control of Biological Hazard Factors (Animal Origin) for Agrifood Safety and Quality, Ministry of Agriculture of China, Yangzhou University, 3Joint International Research Laboratory of Agriculture & Agri-Product Safety, Yangzhou University, 4Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, 5The Testing Center, Yangzhou University

The derivation of a flavonol is crucial for its application in healthcare and the food industry. Here, we provide a detailed protocol for the biosynthesis of a flavonol from a flavanone and discuss the crucial steps and its advantages over other approaches.

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Biology

Measuring Crop Motility and Food Passaging in Drosophila
Jiadong Cai *1,2, Junmeng Xi *1,2, Youheng Wei 1,2
1Joint International Research Laboratory of Agriculture and Agri-Product Safety, the Ministry of Education of China, Yangzhou University, 2College of Bioscience and Biotechnology, Yangzhou University

The goal of this protocol is to measure crop contraction and quantify food distribution in the Drosophila gut.

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Immunology and Infection

Use of Fimbrial Rod for F18ab Fimbriae+ STEC Colonization to Host Cells
Mingxu Zhou *1,2, Qiangde Duan *1,3, Yang Yang 1,3, Guoqiang Zhu 1,3
1College of Veterinary Medicine, Yangzhou University, 2Institute of Veterinary Immunology & Engineering, Jiangsu Academy of Agricultural Sciences, 3Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses

Here we present a protocol to study the function of fimbriae in bacterial colonization.

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Immunology and Infection

A Non-Coding Small RNA MicC Contributes to Virulence in Outer Membrane Proteins in Salmonella Enteritidis
Xia Meng *1,2, Weiwei Cui *1, Xianchen Meng 1, Jianye Wang 1,2, Jinqiu Wang 3, Guoqiang Zhu 1,2
1College of Veterinary Medicine, Yangzhou University, 2Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, 3Department of Animal Husbandry and Veterinary Medicine, Beijing Agricultural Vocational College

An λ-Red-mediated recombination system was used to create a deletion mutant of a small non-coding RNA micC.

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Biology

A Simple Dry Sectioning Method for Obtaining Whole-Seed-Sized Resin Section and Its Applications
Jiajing Qiu *1,2, Yinhui Ren *1,2, Lingxiao Zhao 1,2, Biao Zhang 1,2, Cunxu Wei 1,2
1Key Laboratory of Crop Genetics and Physiology of Jiangsu Province/Jiangsu Key Laboratory of Crop Genomics and Molecular Breeding, Yangzhou University, 2Co-Innovation Center for Modern Production Technology of Grain Crops of Jiangsu Province/Joint International Research Laboratory of Agriculture & Agri-Product Safety of the Ministry of Education, Yangzhou University

This technique allows for the fast and simple preparation of whole-seed-sized resin section for the observation and analysis of cells, starch granules, and protein bodies in different regions of the seed.

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Immunology and Infection

Production of Monoclonal Antibodies Targeting Aminopeptidase N in the Porcine Intestinal Mucosal Epithelium
Pengpeng Xia 1,2,3, Siqi Lian 1,2,3, Yunping Wu 1,2,3, Li Yan 1,2,3
1College of Veterinary Medicine (Institute of comparative medicine), Yangzhou University, 2Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, 3Joint International Research Laboratory of Agriculture and Agri-Product Safety of Ministry of Education of China, Yangzhou University

The recombinant antibody protein expressed in pIRES2-ZSGreen1-rAbs-APN-CHO cells and monoclonal antibodies produced using traditional hybridoma technology can recognize and bind to the porcine aminopeptidase N (APN) protein.

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Biology

A Non-Degradative Extraction Method for Molecular Structure Characterization of Bacterial Glycogen Particles
Jun-Jiao Wang *1, Meng-Meng Wang *2,3, You-Wei He 4, Zhang-Wen Ma 2,3, Zi-Yi Wang 5,6,7, Rui Qiao 2,3, Xin-Le Tan 5,6, Qing-Hua Liu 8, Liang Wang 1,2
1Department of Bioinformatics, School of Medical Informatics and Engineering, Xuzhou Medical University, 2Jiangsu Key Laboratory of New Drug Research and Clinical Pharmacy, School of Pharmacy, Xuzhou Medical University, 3Department of Pharmaceutical Analysis, School of Pharmacy, Xuzhou Medical University, 4Department of Biological Sciences, School of Life Sciences, Xuzhou Medical University, 5School of Chemistry and Molecular Biosciences, The University of Queensland, 6Centre for Nutrition and Food Sciences, Queensland Alliance for Agriculture and Food Innovation, The University of Queensland, 7Joint International Research Laboratory of Agriculture and Agri-Product Safety, College of Agriculture, Yangzhou University, 8State Key Laboratory of Quality Research in Chinese Medicines, Macau University of Science and Technology

Bacterial glycogen structure is greatly impacted by extraction methods which may result in molecular degradation and/or biased sampling. It is essential to develop methods to minimize these problems. Here, four extraction methods have been compared using size distribution and chain length distribution as key criteria for minimizing extraction artifacts.

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Biochemistry

The Extraction of Liver Glycogen Molecules for Glycogen Structure Determination
Ziyi Wang 1,2, Qinghua Liu 3,4, Liang Wang 3,5, Robert G. Gilbert 1,2,6, Mitchell A. Sullivan 7
1School of Chemistry and Molecular Biosciences, The University of Queensland, 2Centre for Nutrition and Food Sciences, Queensland Alliance for Agriculture and Food Innovation, The University of Queensland, 3Jiangsu Provincial Key Laboratory of New Drug Research and Clinical Pharmacy, School of Pharmacy, Xuzhou Medical University, 4Department of Pharmaceutical Analysis, School of Pharmacy, Xuzhou Medical University, 5Department of Bioinformatics, School of Medical Informatics and Engineering, Xuzhou Medical University, 6Joint International Research Laboratory of Agriculture and Agri-Product Safety, College of Agriculture, Yangzhou University, 7Glycation and Diabetes Group, Mater Research Institute-The University of Queensland, Translational Research Institute

An optimal sucrose concentration was determined for the extraction of liver glycogen using sucrose density gradient centrifugation. The addition of a 10 min boiling step to inhibit glycogen-degrading enzymes proved beneficial.

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Developmental Biology

In Ovo Intravascular Injection in Chicken Embryos
Kai Jin 1,2, Jing Zhou 1,2, Gaoyuan Wu 1,2, Ziyi Lian 1,2, Zongyi Zhao 1,2, Shujian Zhou 1,2, Chen Chen 1,2, Hongyan Sun 1,2, Yingjie Niu 1,2, Qishenng Zuo 1,2, Yani Zhang 1,2, Jiuzhou Song 3, Guohong Chen 1,2, Bichun Li 1,2,4
1Joint International Research Laboratory of Agriculture and Agri-Product Safety of Ministry of Education of China, Yangzhou University, 2Key Laboratory of Animal Breeding Reproduction and Molecular Design for Jiangsu Province, College of Animal Science and Technology, Yangzhou University, 3Animal & Avian Sciences, University of Maryland, College Park, 4College of Biotechnology, Jiangsu University of Science and Technology

The overall goal of this paper is to describe how to perform in ovo intracellular injection of exogenous materials into chicken embryos. This approach is very useful to study the developmental biology of chicken embryos.

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Biology

AirID-Based Proximity Labeling for Protein-Protein Interaction in Plants
Ahmad Zada *1, Imran Khan *1, Min Zhang 1, Yuting Cheng 1, Xueyun Hu 1
1College of Bioscience and Biotechnology, Yangzhou University

Here, we present a step-by-step protocol for performing the proximity labeling (PL) experiment in cucumber (Cucumis sativus L.) using AT4G18020 (APRR2)-AirID protein as a model. The method describes the construction of a vector, the transformation of a construct through agroinfiltration, biotin infiltration, protein extraction, and purification of biotin-labeled proteins through affinity purification technique.

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Immunology and Infection

A Mouse Ear Model for Allergic Contact Dermatitis Evaluation
Haifeng Shao 1,2,3,4, Jialin Deng 2,3,4, Yanqing Liu 1,2,3,4, Niuniu Yang 1,2,3,4
1Department of Traditional Chinese Medicine, The Affiliated Hospital of Yangzhou University, Yangzhou University, 2Department of Traditional Chinese and Western Medicine, Medical College, Yangzhou University, 3The Key Laboratory of Syndrome Differentiation and Treatment of Gastric Cancer of the State Administration of Traditional Chinese Medicine, Yangzhou University, 4Jiangsu Key Laboratory of Integrated Traditional Chinese and Western Medicine for Prevention and Treatment of Senile Diseases, Yangzhou University

Here, we describe the methods for inducing allergic contact dermatitis in mouse ears by 1-fluoro-2,4-dinitrobenzene (DNFB) and how to evaluate the severity of allergic contact dermatitis.

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