A Mouse Ear Model for Allergic Contact Dermatitis Evaluation

3.0K Views

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08:02 min

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March 24th, 2023

DOI :

10.3791/65120-v

March 24th, 2023

•

Haifeng Shao¹^,²^,³^,⁴, Jialin Deng²^,³^,⁴, Yanqing Liu¹^,²^,³^,⁴, Niuniu Yang¹^,²^,³^,⁴

¹Department of Traditional Chinese Medicine, The Affiliated Hospital of Yangzhou University, Yangzhou University, ²Department of Traditional Chinese and Western Medicine, Medical College, Yangzhou University, ³The Key Laboratory of Syndrome Differentiation and Treatment of Gastric Cancer of the State Administration of Traditional Chinese Medicine, Yangzhou University, ⁴Jiangsu Key Laboratory of Integrated Traditional Chinese and Western Medicine for Prevention and Treatment of Senile Diseases, Yangzhou University

Summary

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Mouse Ear Model

Allergic Contact Dermatitis

ACD Pathogenesis

Preclinical Evaluation

Therapeutic Methods

Animal Model

Experimental Protocol

DNFB Solution

Sensitization Stimulation

Weight Changes

Ear Sensitization

Pipette Application

Ultraviolet Lamp Disinfection

Acetone Mixture

Abdominal Shaving

Chapters in this video

0:04

Introduction

0:55

Animal Preparation

1:35

Abdominal Sensitization Stimulation

2:46

Ear Sensitization Stimulation

3:38

Recording Mouse Weight and Allergic Contact Dermatitis (ACD) Symptoms

4:16

Measurement of the Auricle Thickness

4:50

Evaluation of the Degree of Inflammatory Swelling

5:59

Results: Evaluating the Severity of Allergic Contact Dermatitis

7:32

Conclusion

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