The Extraction of Liver Glycogen Molecules for Glycogen Structure Determination

4.7K Views

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04:50 min

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February 8th, 2022

DOI :

10.3791/63088-v

February 8th, 2022

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Ziyi Wang¹^,², Qinghua Liu³^,⁴, Liang Wang³^,⁵, Robert G. Gilbert¹^,²^,⁶, Mitchell A. Sullivan⁷

¹School of Chemistry and Molecular Biosciences, The University of Queensland, ²Centre for Nutrition and Food Sciences, Queensland Alliance for Agriculture and Food Innovation, The University of Queensland, ³Jiangsu Provincial Key Laboratory of New Drug Research and Clinical Pharmacy, School of Pharmacy, Xuzhou Medical University, ⁴Department of Pharmaceutical Analysis, School of Pharmacy, Xuzhou Medical University, ⁵Department of Bioinformatics, School of Medical Informatics and Engineering, Xuzhou Medical University, ⁶Joint International Research Laboratory of Agriculture and Agri-Product Safety, College of Agriculture, Yangzhou University, ⁷Glycation and Diabetes Group, Mater Research Institute-The University of Queensland, Translational Research Institute

Summary

Explore More Videos

Glycogen Extraction

Liver Glycogen

Glycogen Structure Determination

Diabetes

Glycogen Storage Disease

Glycogen Isolation Buffer

Tissue Homogenizer

Supernatant Centrifugation

Ethanol Precipitation

Glycogen Yield

Cryopreservation

Purity Analysis

Chapters in this video

0:00

Introduction

0:30

Glycogen Extraction

2:44

Results: The Effect of Sucrose Concentration on the Particle Size of Glycogen

4:25

Conclusion

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