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University Hospital Bonn

8 ARTICLES PUBLISHED IN JoVE

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Medicine

A Murine Closed-chest Model of Myocardial Ischemia and Reperfusion
Se-Chan Kim 1, Olaf Boehm 1, Rainer Meyer 2, Andreas Hoeft 1, Pascal Knüfermann 1, Georg Baumgarten 1
1Department of Anesthesiology and Intensive Care Medicine, University of Bonn, Germany, 2Institute of Physiology, University of Bonn, Germany

Surgical trauma induces an inflammatory response. Cytokines and endogenous ligands are known to modulate myocardial infarct size following ischemia and reperfusion. We present a modified closed-chest model of murine ischemia and reperfusion using hanging weights to minimize effects of thoracotomy.

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Medicine

The Supraclavicular Fossa Ultrasound View for Central Venous Catheter Placement and Catheter Change Over Guidewire
Se-Chan Kim 1, Christian Klebach 1, Ingo Heinze 1, Andreas Hoeft 1, Georg Baumgarten 1, Stefan Weber 1
1Department of Anesthesiology and Intensive Care Medicine, University Hospital Bonn

The overall goal is to enable clinicians to insert a central venous catheter under real-time ultrasound guidance via the right supraclavicular fossa view of the lower part of the superior vena cava. This view is useful for different catheter insertion sites, thrombosis detection before insertion and ultrasound guided correction of misplaced catheters.

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Medicine

A Model to Simulate Clinically Relevant Hypoxia in Humans
Lars Eichhorn 1, Florian Kessler 1, Volker Böhnert 2, Felix Erdfelder 1, Anja Reckendorf 3, Rainer Meyer 4, Richard K. Ellerkmann 1
1Department of Anaesthesiology and Intensive Care Medicine, University Hospital of Bonn, 2Institute of Clinical Chemistry and Clinical Pharmacology, University of Bonn, 3Institute for Terrestrial and Aquatic Wildlife Research, University of Veterinary Medicine Hannover, 4Institute of Physiology 2, University of Bonn

Hypoxia simulation in humans has usually been performed by inhaling hypoxic gas mixtures. For this study, apneic divers were used to simulate dynamic hypoxia in humans. Additionally, physiological changes in desaturation and re-saturation kinetics were evaluated with non-invasive tools such as Near-Infrared-Spectroscopy (NIRS) and peripheral oxygenation saturation (SpO2).

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Medicine

Novel Diagnostics in Revision Arthroplasty: Implant Sonication and Multiplex Polymerase Chain Reaction
Gunnar T.R. Hischebeth 1, Sascha Gravius 2, Johanna K. Buhr 2, Ernst Molitor 1, Matthias D. Wimmer 2, Achim Hoerauf 1, Isabelle Bekeredjian-Ding 3, Thomas M. Randau 2
1Institute of Medical Microbiology, Immunology and Parasitology, University Hospital Bonn, 2Department of Orthopaedics and Trauma Surgery, University Hospital Bonn, 3Division of EU Cooperation/Microbiology, Paul-Ehrlich-Institute

Differential diagnostics in painful arthroplasty is crucial for treatment success. Joint aspiration is routinely performed preoperatively. Multiplex polymerase chain reaction of the joint aspirate and the sonication fluid, a feasible tool for quick pathogen detection from these samples is described.

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Medicine

A Closed-chest Model to Induce Transverse Aortic Constriction in Mice
Lars Eichhorn *1, Christina Katharina Weisheit *1, Christopher Gestrich 2, Konrad Peukert 1, Georg Daniel Duerr 2, Muhammad Ajmal Ayub 3, Felix Erdfelder 1, Florian Stöckigt 4
1Department of Anaesthesiology, University Hospital Bonn, 2Department of Cardiac Surgery, University Hospital Bonn, 3Institute of Physiology 2, University of Bonn, 4Department of Medicine - Cardiology, University Hospital Bonn

Here, we present a protocol of Transverse Aortic constriction (TAC) via a lateral thoracotomy. This technique is a minimally invasive, closed chest surgical procedure aiming to simulate pressure overload and heart failure in mice utilizing standard TAC laboratory settings.

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Medicine

Evaluation of Patients' Posture and Gait Profile After Lumbar Fusion Surgery by Video Rasterstereography and Treadmill Gait Analysis
Sebastian Scheidt 1,2, Ulf Krister Hofmann 2, Falk Mittag 2
1Department of Orthopedics and Trauma Surgery, University Hospital Bonn, 2Department of Orthopedic Surgery, University Hospital Tuebingen

Here, we present a protocol to analyze the posture and the gait of patients after lumbar fusion surgery by means of high-resolution video rasterstereography and a treadmill equipped with an integrated sensor mat. Allowing critical functional postoperative evaluation on a less subjective level may enhance accuracy and reliability of indication for surgery.

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Medicine

Inducing Acute Lung Injury in Mice by Direct Intratracheal Lipopolysaccharide Instillation
Heidi Ehrentraut *1, Christina K. Weisheit *1, Stilla Frede 1, Tobias Hilbert 1
1Department of Anesthesiology and Intensive Care Medicine, University Hospital Bonn

Presented here is a step-by-step procedure to induce acute lung injury in mice by direct intratracheal lipopolysaccharide instillation and to perform FACS analysis of blood samples, bronchoalveolar lavage fluid, and lung tissue. Minimal invasiveness, simple handling, good reproducibility, and titration of disease severity are advantages of this approach.

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Neuroscience

Ex Vivo OCT-Based Multimodal Imaging of Human Donor Eyes for Research into Age-Related Macular Degeneration
Jeffrey D. Messinger 1, Max Brinkmann 2, James A. Kimble 1, Andreas Berlin 1, K. Bailey Freund 3,4, Gregory H. Grossman 5, Thomas Ach *6, Christine A. Curcio *1
1Department of Ophthalmology and Visual Sciences, University of Alabama at Birmingham Heersink School of Medicine, 2Department of Ophthalmology, University Hospital of Zurich, 3Vitreous Retina Macula Consultants of New York, 4Department of Ophthalmology, New York University Grossman School of Medicine, 5Advancing Sight Network, 6Department of Ophthalmology, University Hospital Bonn

Laboratory assays can leverage prognostic value from the longitudinal optical coherence tomography (OCT)-based multimodal imaging of age-related macular degeneration (AMD). Human donor eyes with and without AMD are imaged using OCT, color, near-infrared reflectance scanning laser ophthalmoscopy, and autofluorescence at two excitation wavelengths prior to tissue sectioning.

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