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Université de Bordeaux

6 ARTICLES PUBLISHED IN JoVE

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Neuroscience

Visualization and Genetic Manipulation of Dendrites and Spines in the Mouse Cerebral Cortex and Hippocampus using In utero Electroporation
Emilie Pacary 1, Matilda A. Haas 1, Hendrik Wildner 1, Roberta Azzarelli 1, Donald M. Bell 2, Djoher Nora Abrous 3, François Guillemot 1
1Division of Molecular Neurobiology, MRC National Institute for Medical Research, 2Confocal and Image Analysis Laboratory, National Institute for Medical Research, 3Physiopathologie de la plasticité neuronale, Neurocentre Magendie, Université de Bordeaux

This article describes in detail a protocol to electroporate in utero the cerebral cortex and the hippocampus at E14.5 in mice. We also show that this is a valuable method to study dendrites and spines in these two cerebral regions.

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Biology

Plunge Freezing: A Tool for the Ultrastructural and Immunolocalization Studies of Suspension Cells in Transmission Electron Microscopy
Corinne Blancard 1, Bénédicte Salin 1
1Institut de Biochimie et Génétique Cellulaires, Centre National de la Recherche Scientifique, UMR 5095, Université de Bordeaux

This manuscript describes an easy-to-use and low-cost cryofixation method for visualizing suspension cells by transmission electron microscopy.

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Chemistry

In Situ Detection and Single Cell Quantification of Metal Oxide Nanoparticles Using Nuclear Microprobe Analysis
Giovanna Muggiolu *1,2, Marina Simon *1,2, Nathanael Lampe 1,2, Guillaume Devès 1,2, Philippe Barberet 1,2, Claire Michelet 1,2, Marie-Hélène Delville 3,4, Hervé Seznec 1,2
1Centre d'Etudes Nucléaires Bordeaux Gradignan (CENBG), Université de Bordeaux, 2Centre d'Etudes Nucléaires Bordeaux Gradignan (CENBG), CNRS, 3Institut de Chimie de la Matière Condens é e de Bordeaux (ICMCB), CNRS, 4Institut de Chimie de la Matière Condens é e de Bordeaux (ICMCB), Université de Bordeaux

We describe a procedure for the detection of chemical elements present in situ in human cells as well as their in vitro quantification. The method is well-suited to any cell type and is particularly useful for quantitative chemical analyses in single cells following in vitro metal oxide nanoparticles exposure.

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Chemistry

Photogeneration of N-Heterocyclic Carbenes: Application in Photoinduced Ring-Opening Metathesis Polymerization
Julien Pinaud 1, Emeline Placet 1, Patrick Lacroix-Desmazes 1, Thi Kim Hoang Trinh 2,3, Jean Pierre Malval 2,3, Abraham Chemtob 2,3, Loïc Pichavant 4, Valérie Héroguez 4
1ICGM, Université de Montpellier, CNRS, ENSCM, 2Institut de Science des Matériaux de Mulhouse (IS2M UMR 7361 CNRS), Université de Haute-Alsace, 3Université de Strasbourg, 4Laboratoire de Chimie des Polymères Organiques (LCPO UMR 5629 ENSCBP), Université de Bordeaux

We describe a protocol to photogenerate N-heterocyclic carbenes (NHCs) by UV irradiation of a 2-isopropylthioxanthone/imidazolium tetraphenylborate salt system. Methods to characterize the photoreleased NHC and elucidate the photochemical mechanism are proposed. The protocols for ring-opening metathesis photopolymerization in solution and miniemulsion illustrate the potential of this 2-component NHC photogenerating system.

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Behavior

Handling Techniques to Reduce Stress in Mice
Michael Marcotte 1, Ashley Bernardo 1,2, Nathaniel Linga 3, Carmina A. Pérez-Romero 4, Jean-Louis Guillou 5, Etienne Sibille 1,2,3, Thomas D. Prevot 1,2
1Campbell Family Mental Health Research Institute of CAMH, 2Department of Psychiatry, University of Toronto, 3Department of Pharmacology and Toxicology, University of Toronto, 4Departamento de Investigación, Universidad Central de Queretaro, 5Centre National de la Recherche Scientifique, UMR 5287, Institut de Neurosciences Cognitives et Intégratives d'Aquitaine, Université de Bordeaux

This paper describes a handling technique in mice, the 3D-handling technique, which facilitates routine handling by reducing anxiety-like behaviors and presents details on two existing related techniques (tunnel and tail handling).

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Biochemistry

High-Resolution Neutron Spectroscopy to Study Picosecond-Nanosecond Dynamics of Proteins and Hydration Water
Kevin Pounot 1,2, Markus Appel 2, Christian Beck 1,2, Martin Weik 3, Giorgio Schirò 3, Yann Fichou 4, Tilo Seydel 2, Frank Schreiber 1
1Institut für Angewandte Physik, Universität Tübingen, 2Institut Max von Laue - Paul Langevin (ILL), 3Institut de Biologie Structurale, Université Grenoble Alpes, 4Institut Européen de Chimie et Biologie, Bordeaux INP, Chimie et Biologie des Membranes et des Nanoobjets (CBMN), Université de Bordeaux

Neutron backscattering spectroscopy offers a nondestructive and label-free access to the ps-ns dynamics of proteins and their hydration water. The workflow is presented with two studies on amyloid proteins: on the time-resolved dynamics of lysozyme during aggregation and on the hydration water dynamics of tau upon fiber formation.

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