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Plunge Freezing: A Tool for the Ultrastructural and Immunolocalization Studies of Suspension Cells in Transmission Electron Microscopy

10.8K Views

13:35 min

May 5th, 2017

DOI :

10.3791/54874-v

May 5th, 2017


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Keywords Plunge Freezing

Chapters in this video

0:00

Title

1:38

Preparation of Formvar Grid Film

3:53

Preparation of Freeze-substitution Medium

5:06

Preparation of Cells

6:11

Liquefaction of Propane

7:32

Plunge Freezing of Samples

8:32

Freeze-substitution

9:04

Sample Warming

10:57

Results: Plunge Freezing Results in Improved Preservation of the Intracellular Structures

12:50

Conclusion

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