Chongqing Medical University

3 ARTICLES PUBLISHED IN JoVE

Immunology and Infection

Draining Lymph Node Metastasis Model for Assessing the Dynamics of Antigen-Specific CD8⁺ T Cells During Tumorigenesis

Yan Zhang^*1, Xingxing Su^*2, Lisha Wang², Zhengliang Yue², Qiao Liu³, Ling Ran², Shun Lei², Jianjun Hu², Lifan Xu², Lilin Ye², Ping Ji⁴, Guimei Li¹, Qizhao Huang⁵, Shuqiong Wen⁴

¹College of Veterinary Medicine, Qingdao Agricultural University, ²Institute of Immunology, Third Military Medical University, ³Institute of Cancer, Xinqiao Hospital, Third Military Medical University, ⁴Stomatological Hospital of Chongqing Medical University, ⁵Institute of life science, Chongqing Medical University

The experimental design presented here provides a useful reproductive model for the studies of antigen-specific CD8⁺ T cells during lymph node (LN) metastasis, which excludes the perturbation of bystander CD8⁺ T cells.

Developmental Biology

Generation, Maintenance, and Identification of Germ-Free Zebrafish Models from Larvae to Juvenile Stages

Pan-Pan Jia¹, Xiang-Ru Liu¹, Ming-Fei Wu¹, Yan Li¹, Lan-Chen Zhang¹, De-Sheng Pei¹

¹School of Public Health, Chongqing Medical University

This protocol outlines the primary steps for obtaining germ-free (GF) fish embryos and maintaining them from larvae until the juvenile stage, including sampling and detecting their sterile status. The use of GF models with infection is important for understanding the role of microbes in host health.

Immunology and Infection

Accessing Early Differentiation of Virus-Specific Follicular Helper CD4⁺ T Cell in Acute LCMV-Infected Mice

Yao Lin^*1,2, Shuai Yue^*3, Yang Yang^*4, Junjian He⁵, Xiaofan Yang⁶, Lilin Ye⁵, Xiangyu Chen⁷

¹Department of Urology, South China Hospital, Medical School, Shenzhen University, ²Guangdong Key Laboratory for Biomedical Measurements and Ultrasound Imaging, National-Regional Key Technology Engineering Laboratory for Medical Ultrasound, School of Biomedical Engineering, Shenzhen University Medical School, ³Cancer Center, Daping Hospital & Army Medical Center of PLA, Third Military Medical University, ⁴Guangdong Provincial Key Laboratory of Immune Regulation and Immunotherapy, School of Laboratory Medicine and Biotechnology, Southern Medical University, ⁵Institute of Immunology, Third Military Medical University, ⁶Dermatology Hospital, Southern Medical University, ⁷Institute of Immunological Innovation and Translation, Chongqing Medical University

The current study showcases protocols for assessing the early fate commitment of virus-specific T_FH cells and manipulating gene expression in these cells.