Accedi

Chongqing Medical University

4 ARTICLES PUBLISHED IN JoVE

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Immunology and Infection

Draining Lymph Node Metastasis Model for Assessing the Dynamics of Antigen-Specific CD8+ T Cells During Tumorigenesis
Yan Zhang *1, Xingxing Su *2, Lisha Wang 2, Zhengliang Yue 2, Qiao Liu 3, Ling Ran 2, Shun Lei 2, Jianjun Hu 2, Lifan Xu 2, Lilin Ye 2, Ping Ji 4, Guimei Li 1, Qizhao Huang 5, Shuqiong Wen 4
1College of Veterinary Medicine, Qingdao Agricultural University, 2Institute of Immunology, Third Military Medical University, 3Institute of Cancer, Xinqiao Hospital, Third Military Medical University, 4Stomatological Hospital of Chongqing Medical University, 5Institute of life science, Chongqing Medical University

The experimental design presented here provides a useful reproductive model for the studies of antigen-specific CD8+ T cells during lymph node (LN) metastasis, which excludes the perturbation of bystander CD8+ T cells.

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Medicine

Enhancement of Facial Rejuvenation Through a Combination of 1565 nm Non-Ablative Fractional Laser with 30% Supramolecular Salicylic Acid
Qianwen Zeng 1, Qian Wu 1, Li Deng 1, Liangjian Hong 2, Ruiqi Li 3, Aijun Chen 1
1Department of Dermatology, The First Affiliated Hospital, Chongqing Medical University, 2Department of Dermatology, Chongqing Contemporary Plastic Surgery Hospital, 3Department of Dermatology, Chongqing Huamei Plastic Surgery Hospital

The purpose of this project is to effectively reduce the occurrence of adverse reactions such as erythema and pigmentation after non-ablative fractional laser treatment by combining 1565 non-ablative fractional laser with 30% super molecular salicylic acid, providing patients with a safer new method of facial rejuvenation treatment.

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Developmental Biology

Generation, Maintenance, and Identification of Germ-Free Zebrafish Models from Larvae to Juvenile Stages
Pan-Pan Jia 1, Xiang-Ru Liu 1, Ming-Fei Wu 1, Yan Li 1, Lan-Chen Zhang 1, De-Sheng Pei 1
1School of Public Health, Chongqing Medical University

This protocol outlines the primary steps for obtaining germ-free (GF) fish embryos and maintaining them from larvae until the juvenile stage, including sampling and detecting their sterile status. The use of GF models with infection is important for understanding the role of microbes in host health.

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Immunology and Infection

Accessing Early Differentiation of Virus-Specific Follicular Helper CD4+ T Cell in Acute LCMV-Infected Mice
Yao Lin *1,2, Shuai Yue *3, Yang Yang *4, Junjian He 5, Xiaofan Yang 6, Lilin Ye 5, Xiangyu Chen 7
1Department of Urology, South China Hospital, Medical School, Shenzhen University, 2Guangdong Key Laboratory for Biomedical Measurements and Ultrasound Imaging, National-Regional Key Technology Engineering Laboratory for Medical Ultrasound, School of Biomedical Engineering, Shenzhen University Medical School, 3Cancer Center, Daping Hospital & Army Medical Center of PLA, Third Military Medical University, 4Guangdong Provincial Key Laboratory of Immune Regulation and Immunotherapy, School of Laboratory Medicine and Biotechnology, Southern Medical University, 5Institute of Immunology, Third Military Medical University, 6Dermatology Hospital, Southern Medical University, 7Institute of Immunological Innovation and Translation, Chongqing Medical University

The current study showcases protocols for assessing the early fate commitment of virus-specific TFH cells and manipulating gene expression in these cells.

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