Illinois State University

4 ARTICLES PUBLISHED IN JoVE

Biochemistry

Detection of the pH-dependent Activity of Escherichia coli Chaperone HdeB In Vitro and In Vivo

Jan-Ulrik Dahl¹, Philipp Koldewey^1,2, James C. A. Bardwell^1,2, Ursula Jakob¹

¹Department of Molecular, Cellular, and Developmental Biology, University of Michigan, ²Howard Hughes Medical Institute, University of Michigan

This study describes biophysical, biochemical and molecular techniques to characterize the chaperone activity of Escherichia coli HdeB under acidic pH conditions. These methods have been successfully applied for other acid-protective chaperones such as HdeA and can be modified to work for other chaperones and stress conditions.

Behavior

Measuring Biophysical and Psychological Stress Levels Following Visitation to Three Locations with Differing Levels of Nature

Yun Chang¹, Alan Ewert², Lisa M. Kamendulis³, Barbara A. Hocevar³

¹School of Kinesiology and Recreation, Illinois State University, ²Department of Recreation, Park, and Tourism Studies, Indiana University, ³Department pf Environmental and Occupational Health, School of Public Health-Bloomington, Indiana University

The purpose of this paper is to identify changes in stress levels after visitation to three different settings and to describe the methods used in identifying stress levels based on measures of salivary cortisol, α-amylase, and a psychological self-report instrument.

Biochemistry

Extraction and Visualization of Protein Aggregates after Treatment of Escherichia coli with a Proteotoxic Stressor

Sadia Sultana¹, Greg M. Anderson¹, Kevin Pierre Hoffmann¹, Jan-Ulrik Dahl¹

¹School of Biological Sciences, Illinois State University

This protocol describes the extraction and visualization of aggregated and soluble proteins from Escherichia coli after treatment with a proteotoxic antimicrobial. Following this procedure allows a qualitative comparison of protein aggregate formation in vivo in different bacterial strains and/or between treatments.

Immunology and Infection

Rose Bengal-Mediated Photodynamic Therapy to Inhibit Candida albicans

Jia-Horung Hung^*1,2, Zhao-Xiang Wang^*3,4, Yuan-Hsin Lo⁵, Chaw-Ning Lee^3,6, Yun Chang⁷, Rita Yuchiao Chang⁷, Chien-Chun Huang³, Tak-Wah Wong^3,8,9

The growing incidence of drug-resistant Candida albicans is a serious health issue worldwide. Antimicrobial photodynamic therapy (aPDT) may offer a strategy to fight against drug-resistant fungal infections. The present protocol describes Rose bengal-mediated aPDT efficacy on a multidrug-resistant C. albicans strain in vitro.