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Illinois State University

4 ARTICLES PUBLISHED IN JoVE

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Biochemistry

Detection of the pH-dependent Activity of Escherichia coli Chaperone HdeB In Vitro and In Vivo
Jan-Ulrik Dahl 1, Philipp Koldewey 1,2, James C. A. Bardwell 1,2, Ursula Jakob 1
1Department of Molecular, Cellular, and Developmental Biology, University of Michigan, 2Howard Hughes Medical Institute, University of Michigan

This study describes biophysical, biochemical and molecular techniques to characterize the chaperone activity of Escherichia coli HdeB under acidic pH conditions. These methods have been successfully applied for other acid-protective chaperones such as HdeA and can be modified to work for other chaperones and stress conditions.

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Behavior

Measuring Biophysical and Psychological Stress Levels Following Visitation to Three Locations with Differing Levels of Nature
Yun Chang 1, Alan Ewert 2, Lisa M. Kamendulis 3, Barbara A. Hocevar 3
1School of Kinesiology and Recreation, Illinois State University, 2Department of Recreation, Park, and Tourism Studies, Indiana University, 3Department pf Environmental and Occupational Health, School of Public Health-Bloomington, Indiana University

The purpose of this paper is to identify changes in stress levels after visitation to three different settings and to describe the methods used in identifying stress levels based on measures of salivary cortisol, α-amylase, and a psychological self-report instrument. 

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Biochemistry

Extraction and Visualization of Protein Aggregates after Treatment of Escherichia coli with a Proteotoxic Stressor
Sadia Sultana 1, Greg M. Anderson 1, Kevin Pierre Hoffmann 1, Jan-Ulrik Dahl 1
1School of Biological Sciences, Illinois State University

This protocol describes the extraction and visualization of aggregated and soluble proteins from Escherichia coli after treatment with a proteotoxic antimicrobial. Following this procedure allows a qualitative comparison of protein aggregate formation in vivo in different bacterial strains and/or between treatments.

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Immunology and Infection

Rose Bengal-Mediated Photodynamic Therapy to Inhibit Candida albicans
Jia-Horung Hung *1,2, Zhao-Xiang Wang *3,4, Yuan-Hsin Lo 5, Chaw-Ning Lee 3,6, Yun Chang 7, Rita Yuchiao Chang 7, Chien-Chun Huang 3, Tak-Wah Wong 3,8,9
1Institute of Clinical Medicine, College of Medicine, National Cheng Kung University, 2Department of Ophthalmology, National Cheng Kung University Hospital, College of Medicine, National Cheng Kung University, 3Department of Dermatology, National Cheng Kung University Hospital, College of Medicine, National Cheng Kung University, 4Department of Microbiology and Immunology, College of Medicine, National Cheng Kung University, 5Department of Dermatology, Fu Jen Catholic University Hospital, Fu Jen Catholic University, 6Institute of Clinical Pharmacy and Pharmaceutical Sciences, College of Medicine, National Cheng Kung University, 7School of Medicine, National Cheng Kung University, 8Department of Biochemistry and Molecular Biology, College of Medicine, National Cheng Kung University, 9Center of Applied Nanomedicine, National Cheng Kung University

The growing incidence of drug-resistant Candida albicans is a serious health issue worldwide. Antimicrobial photodynamic therapy (aPDT) may offer a strategy to fight against drug-resistant fungal infections. The present protocol describes Rose bengal-mediated aPDT efficacy on a multidrug-resistant C. albicans strain in vitro.

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