A Step-By-Step Method to Detect Neutralizing Antibodies Against AAV using a Colorimetric Cell-Based Assay

4.4K Views

•

05:53 min

•

December 7th, 2021

DOI :

10.3791/63419-v

December 7th, 2021

•

Sebastian Bass-Stringer¹^,², Colleen J. Thomas²^,³, Clive N. May³, Paul Gregorevic⁴, Kate L. Weeks¹^,⁵^,⁶, Julie R. McMullen¹^,²^,⁵^,⁶^,⁷

¹Baker Heart and Diabetes Institute, ²Department of Physiology, Anatomy and Microbiology, La Trobe University, ³Florey Institute of Neuroscience and Mental Health, University of Melbourne, ⁴Department of Physiology, Centre for Muscle Research (CMR), The University of Melbourne, ⁵Department of Diabetes, Central Clinical School, Monash University, ⁶Baker Department of Cardiometabolic Health, The University of Melbourne, ⁷Department of Physiology and Department of Medicine Alfred Hospital, Monash University

Summary

Explore More Videos

Neutralizing Antibodies

Chapters in this video

0:05

Introduction

0:36

Plating and Infecting HT1080 Cells

2:00

Day 3: Fixing and Adding Substrate to The Cells

3:04

Quantitation to Determine the Neutralizing Activity Using ImageJ

3:48

Results: Transduction Assay and Assessment of Neutralizing Antibodies (Nabs) Against AAV6 in Sheep Samples

5:26

Conclusion

Related Videos

17:03

Direct Observation of Enzymes Replicating DNA Using a Single-molecule DNA Stretching Assay

18.7K Views

11:02

Chromatin Immunoprecipitation Assay for Tissue-specific Genes using Early-stage Mouse Embryos

18.0K Views

12:25

A Step-by-step Method for the Reconstitution of an ABC Transporter into Nanodisc Lipid Particles

25.3K Views

14:48

Conversion of a Capture ELISA to a Luminex xMAP Assay using a Multiplex Antibody Screening Method

43.5K Views

08:29

Using Coculture to Detect Chemically Mediated Interspecies Interactions

13.5K Views

08:20

Analysis of Apoptosis in Zebrafish Embryos by Whole-mount Immunofluorescence to Detect Activated Caspase 3

19.8K Views

12:40

A Method for Screening and Validation of Resistant Mutations Against Kinase Inhibitors

14.8K Views

09:39

Quantitative Immunofluorescence Assay to Measure the Variation in Protein Levels at Centrosomes

14.9K Views

12:31

A Method for Selecting Structure-switching Aptamers Applied to a Colorimetric Gold Nanoparticle Assay

15.0K Views

10:05

Colorimetric Detection of Bacteria Using Litmus Test

9.1K Views