JoVE Logo

Sign In

Functional Assessment of BRCA1 variants using CRISPR-Mediated Base Editors

5.4K Views

09:22 min

February 28th, 2021

DOI :

10.3791/61557-v

February 28th, 2021


Explore More Videos

CRISPR

Chapters in this video

0:05

Introduction

1:03

Design and Construction of BRCA1 Targeting gRNAs

3:17

Creation of BRCA1 Variants using CRISPR-mediated Base Editing Tools

4:15

Sample Preparation for Illumina Next-Generation Sequencing (NGS)

7:01

Results: Functional Study of BRCA1 using CRISPR-Cas9 Systems

8:34

Conclusion

Related Videos

article

08:25

Using a Fluorescent PCR-capillary Gel Electrophoresis Technique to Genotype CRISPR/Cas9-mediated Knockout Mutants in a High-throughput Format

13.7K Views

article

10:07

A Standard Methodology to Examine On-site Mutagenicity As a Function of Point Mutation Repair Catalyzed by CRISPR/Cas9 and SsODN in Human Cells

7.7K Views

article

07:56

Genome Editing in Mammalian Cell Lines using CRISPR-Cas

21.4K Views

article

07:46

CRISPR/Cas9 Editing of the C. elegans rbm-3.2 Gene using the dpy-10 Co-CRISPR Screening Marker and Assembled Ribonucleoprotein Complexes.

5.7K Views

article

08:23

CIRCLE-Seq for Interrogation of Off-Target Gene Editing

431 Views

article

08:53

Identifying the Effects of BRCA1 Mutations on Homologous Recombination using Cells that Express Endogenous Wild-type BRCA1

14.6K Views

article

08:15

gDNA Enrichment by a Transposase-based Technology for NGS Analysis of the Whole Sequence of BRCA1, BRCA2, and 9 Genes Involved in DNA Damage Repair

12.2K Views

article

10:40

CRISPR Gene Editing Tool for MicroRNA Cluster Network Analysis

2.3K Views

article

07:31

Efficient PAM-Less Base Editing for Zebrafish Modeling of Human Genetic Disease with zSpRY-ABE8e

1.1K Views

article

02:01

CRISPR-Mediated Base Editing Tools: A Genome Editing Technique to Induce Targeted Base Substitution

1.4K Views

JoVE Logo

Privacy

Terms of Use

Policies

Research

Education

ABOUT JoVE

Copyright © 2025 MyJoVE Corporation. All rights reserved